| Jurisdiction: | Diocese |
| Cienfuegos | |
| Latin: | Dioecesis Centumfocencis |
| Country: |  Cuba |
| Metropolitan: | Cienfuegos |
| Province: | Province of Camagüey |
| Area Km2: | 5,360 |
| Population: | 485,000 |
| Population As Of: | 2006 |
| Catholics: | 293,600 |
| Catholics Percent: | 60.5 |
| Parishes: | 22 |
| Denomination: | Roman Catholic |
| Rite: | Latin Rite |
| Established: | 20 February 1903 (years ago) |
| Cathedral: | Cathedral of Our Lady of the Immaculate Conception |
| Bishop Title: | Bishop |
| Map: | Diócesis de Cienfuegos.svg |
The Roman Catholic Diocese of Cienfuegos is a suffragan diocese of the Archdiocese of Camagüey. The original Diocese of Cienfuegos was erected in 1903 and renamed as the Diocese of Cienfuegos-Santa Clara in 1971. That diocese was split in 1995 to form the Dioceses of Cienfuegos and Santa Clara.