Ribonuclease Z Explained
| TRNase Z |
| Ec Number: | 3.1.26.11 |
| Cas Number: | 98148-84-6 |
TRNase Z (3 tRNase, tRNA 3 endonuclease, RNase Z, 3' tRNase) is an enzyme[1] [2] [3] [4] [5] [6] [7] that, among other things, catalyses the reactions involved in the maturation of tRNAs. Here, it endonucleolytically cleaves the RNA and removes extra 3' nucleotides from the tRNA precursor, generating the 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule. Similarly, it processes tRNA-like molecules such as mascRNA.[8]
Notes and References
- Schiffer S, Rösch S, Marchfelder A . Assigning a function to a conserved group of proteins: the tRNA 3'-processing enzymes . The EMBO Journal . 21 . 11 . 2769–77 . June 2002 . 12032089 . 126033 . 10.1093/emboj/21.11.2769 .
- Mayer M, Schiffer S, Marchfelder A . tRNA 3' processing in plants: nuclear and mitochondrial activities differ . Biochemistry . 39 . 8 . 2096–105 . February 2000 . 10684660 . 10.1021/bi992253e .
- Schiffer S, Helm M, Théobald-Dietrich A, Giegé R, Marchfelder A . The plant tRNA 3' processing enzyme has a broad substrate spectrum . Biochemistry . 40 . 28 . 8264–72 . July 2001 . 11444972 . 10.1021/bi0101953 .
- Kunzmann A, Brennicke A, Marchfelder A . 5' end maturation and RNA editing have to precede tRNA 3' processing in plant mitochondria . Proceedings of the National Academy of Sciences of the United States of America . 95 . 1 . 108–13 . January 1998 . 9419337 . 18142 . 10.1073/pnas.95.1.108 . free .
- Mörl M, Marchfelder A . The final cut. The importance of tRNA 3'-processing . EMBO Reports . 2 . 1 . 17–20 . January 2001 . 11252717 . 1083803 . 10.1093/embo-reports/kve006 .
- Minagawa A, Takaku H, Takagi M, Nashimoto M . A novel endonucleolytic mechanism to generate the CCA 3' termini of tRNA molecules in Thermotoga maritima . The Journal of Biological Chemistry . 279 . 15 . 15688–97 . April 2004 . 14749326 . 10.1074/jbc.M313951200 . free .
- Takaku H, Minagawa A, Takagi M, Nashimoto M . A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease . Nucleic Acids Research . 31 . 9 . 2272–8 . May 2003 . 12711671 . 154223 . 10.1093/nar/gkg337 .
- Wilusz JE, Freier SM, Spector DL . 3' end processing of a long nuclear-retained noncoding RNA yields a tRNA-like cytoplasmic RNA . Cell . 135 . 5 . 919–32 . November 2008 . 19041754 . 2722846 . 10.1016/j.cell.2008.10.012 .
