Repression of heat shock gene expression (ROSE) element explained

Repression of heat shock gene expression (ROSE) element
Symbol:	ROSE
Rfam:	RF00435
Rna Type:	Cis-reg thermoregulator
Tax Domain:	Bacteria

The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.^{[1] [2]}

A partial structure of this RNA element has been determined using NMR.

ROSE₁ and ROSE_AT2

ROSE₁ and ROSE_AT2 are specific examples of ROSE elements.^[3] ROSE₁ is found in Bradyrhizobium japonicum whereas ROSE_AT2 is a closely related element from Agrobacterium tumefaciens. The two RNA elements have similar secondary structures with ROSE₁ having an extra hairpin. All ROSE elements contain a characteristic 'bulged G' opposite the Shine-Dalgarno sequence binding site, without this nucleotide the RNA thermometer loses its temperature-sensitivity.^[4]

IpbA

Expression of the heat shock protein IpbA in species of Pseudomonas is controlled by a ROSE-type RNA thermometer. This thermometer only consists of two hairpins. It inhibits translation of IpbA at low temperatures and permits translation when temperature increases.^[5]

rhlA and lsdI ROSE-like elements

Pseudomonas aeruginosa quorum sensing-dependent virulence factors can be thermoregulated. ROSE thermometer present in the 5'UTR of rhlA and ROSE-like thermometer in the 5'UTR of lasI block the translation at lower temperatures.^[6]

See also

• Cyanobacterial RNA thermometer
• FourU thermometer
• Hsp90 cis-regulatory element

Further reading

• De La Fuente . M. . Valera . S. . Martínez-Guitarte . J. L. . 10.1016/j.febslet.2012.10.018 . NcRNAs and thermoregulation: A view in prokaryotes and eukaryotes . FEBS Letters . 586 . 23 . 4061–4069 . 2012 . 23098758 . free .

Notes and References

1. Nocker . A . Hausherr T . Balsiger S . Krstulovic NP . Hennecke H . Narberhaus F . 2001 . A mRNA-based thermosensor controls expression of rhizobial heat shock genes . Nucleic Acids Res . 29 . 4800–4807 . 11726689 . 10.1093/nar/29.23.4800 . 23 . 96696.
2. Balsiger . S . Ragaz C . Baron C . Narberhaus F . 2004 . Replicon-Specific Regulation of Small Heat Shock Genes in Agrobacterium tumefaciens . J Bacteriol . 186 . 6824–6829 . 15466035 . 10.1128/JB.186.20.6824-6829.2004 . 20 . 522190.
3. Narberhaus F, Waldminghaus T, Chowdhury S . RNA thermometers . FEMS Microbiol. Rev. . 30 . 1 . 3–16 . January 2006 . 16438677 . 10.1111/j.1574-6976.2005.004.x .
4. Nocker A, Hausherr T, Balsiger S, Krstulovic NP, Hennecke H, Narberhaus F . A mRNA-based thermosensor controls expression of rhizobial heat shock genes . Nucleic Acids Res. . 29 . 23 . 4800–4807 . December 2001 . 11726689 . 96696 . 10.1093/nar/29.23.4800.
5. Krajewski. SS. Nagel, M . Narberhaus, F . Short ROSE-Like RNA Thermometers Control IbpA Synthesis in Pseudomonas Species.. PLOS ONE. 2013. 8. 5. e65168. 23741480. 10.1371/journal.pone.0065168. 3669281. 2013PLoSO...865168K. free.
6. Grosso-Becerra. María Victoria. Croda-García. Gerardo. Merino. Enrique. Servín-González. Luis. Mojica-Espinosa. Raúl. Soberón-Chávez. Gloria. 2014-10-28. Regulation of Pseudomonas aeruginosa virulence factors by two novel RNA thermometers. Proceedings of the National Academy of Sciences of the United States of America. 111. 43. 15562–15567. 10.1073/pnas.1402536111. 1091-6490. 4217398. 25313031. 2014PNAS..11115562G. free.