Replication protein A2 explained

Replication protein A 32 kDa subunit is a protein that in humans is encoded by the RPA2 gene.^{[1] [2]}

Interactions

RPA2 has been shown to interact with:

• Cyclin O,^[3]
• DNA-PKcs,
• Ku70,^[4]
• MEN1,^[5]
• RPA3,^[6]
• Replication protein A1,^{[4] [7]}
• STAT3,^[8]
• TP53BP1^[9] and
• Uracil-DNA glycosylase.^[10]

See also

• Single-stranded binding protein
• Replication protein A
• Replication protein A1
• Replication protein A3

Further reading

• Dutta A, Stillman B . cdc2 family kinases phosphorylate a human cell DNA replication factor, RPA, and activate DNA replication . The EMBO Journal . 11 . 6 . 2189–99 . Jun 1992 . 1318195 . 556686 . 10.1002/j.1460-2075.1992.tb05278.x.
• Erdile LF, Wold MS, Kelly TJ . The primary structure of the 32-kDa subunit of human replication protein A . The Journal of Biological Chemistry . 265 . 6 . 3177–82 . Feb 1990 . 10.1016/S0021-9258(19)39750-9 . 2406247 . free .
• Li L, Lu X, Peterson CA, Legerski RJ . An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair . Molecular and Cellular Biology . 15 . 10 . 5396–402 . Oct 1995 . 7565690 . 230789 . 10.1128/mcb.15.10.5396.
• Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ . High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3) . Genomics . 20 . 2 . 249–57 . Mar 1994 . 8020972 . 10.1006/geno.1994.1161 . free .
• Nagelhus TA, Haug T, Singh KK, Keshav KF, Skorpen F, Otterlei M, Bharati S, Lindmo T, Benichou S, Benarous R, Krokan HE . A sequence in the N-terminal region of human uracil-DNA glycosylase with homology to XPA interacts with the C-terminal part of the 34-kDa subunit of replication protein A . The Journal of Biological Chemistry . 272 . 10 . 6561–6 . Mar 1997 . 9045683 . 10.1074/jbc.272.10.6561 . free .
• Amacker M, Hottiger M, Mossi R, Hübscher U . HIV-1 nucleocapsid protein and replication protein A influence the strand displacement DNA synthesis of lentiviral reverse transcriptase . AIDS . 11 . 4 . 534–6 . Mar 1997 . 9084803 .
• Niu H, Erdjument-Bromage H, Pan ZQ, Lee SH, Tempst P, Hurwitz J . Mapping of amino acid residues in the p34 subunit of human single-stranded DNA-binding protein phosphorylated by DNA-dependent protein kinase and Cdc2 kinase in vitro . The Journal of Biological Chemistry . 272 . 19 . 12634–41 . May 1997 . 9139719 . 10.1074/jbc.272.19.12634 . free .
• Zernik-Kobak M, Vasunia K, Connelly M, Anderson CW, Dixon K . Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells . The Journal of Biological Chemistry . 272 . 38 . 23896–904 . Sep 1997 . 9295339 . 10.1074/jbc.272.38.23896 . free .
• Bochkareva E, Frappier L, Edwards AM, Bochkarev A . The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain . The Journal of Biological Chemistry . 273 . 7 . 3932–6 . Feb 1998 . 9461578 . 10.1074/jbc.273.7.3932 . free .
• Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y . Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes . The EMBO Journal . 18 . 5 . 1397–406 . Mar 1999 . 10064605 . 1171229 . 10.1093/emboj/18.5.1397 .
• Otterlei M, Warbrick E, Nagelhus TA, Haug T, Slupphaug G, Akbari M, Aas PA, Steinsbekk K, Bakke O, Krokan HE . Post-replicative base excision repair in replication foci . The EMBO Journal . 18 . 13 . 3834–44 . Jul 1999 . 10393198 . 1171460 . 10.1093/emboj/18.13.3834 .
• Bochkarev A, Bochkareva E, Frappier L, Edwards AM . The crystal structure of the complex of replication protein A subunits RPA32 and RPA14 reveals a mechanism for single-stranded DNA binding . The EMBO Journal . 18 . 16 . 4498–504 . Aug 1999 . 10449415 . 1171524 . 10.1093/emboj/18.16.4498 .
• Lao Y, Gomes XV, Ren Y, Taylor JS, Wold MS . Replication protein A interactions with DNA. III. Molecular basis of recognition of damaged DNA . Biochemistry . 39 . 5 . 850–9 . Feb 2000 . 10653628 . 10.1021/bi991704s .
• Costello JF, Frühwald MC, Smiraglia DJ, Rush LJ, Robertson GP, Gao X, Wright FA, Feramisco JD, Peltomäki P, Lang JC, Schuller DE, Yu L, Bloomfield CD, Caligiuri MA, Yates A, Nishikawa R, Su Huang H, Petrelli NJ, Zhang X, O'Dorisio MS, Held WA, Cavenee WK, Plass C . Aberrant CpG-island methylation has non-random and tumour-type-specific patterns . Nature Genetics . 24 . 2 . 132–8 . Feb 2000 . 10655057 . 10.1038/72785 . 6273676 .
• Kim J, Kim D, Chung J . Replication protein a 32 kDa subunit (RPA p32) binds the SH2 domain of STAT3 and regulates its transcriptional activity . Cell Biology International . 24 . 7 . 467–73 . 2000 . 10875894 . 10.1006/cbir.2000.0525 . 23783745 .
• Cho JM, Song DJ, Bergeron J, Benlimame N, Wold MS, Alaoui-Jamali MA . RBT1, a novel transcriptional co-activator, binds the second subunit of replication protein A . Nucleic Acids Research . 28 . 18 . 3478–85 . Sep 2000 . 10982866 . 110737 . 10.1093/nar/28.18.3478 .
• Brush GS, Kelly TJ . Phosphorylation of the replication protein A large subunit in the Saccharomyces cerevisiae checkpoint response . Nucleic Acids Research . 28 . 19 . 3725–32 . Oct 2000 . 11000264 . 110765 . 10.1093/nar/28.19.3725 .
• Mer G, Bochkarev A, Gupta R, Bochkareva E, Frappier L, Ingles CJ, Edwards AM, Chazin WJ . Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA . Cell . 103 . 3 . 449–56 . Oct 2000 . 11081631 . 10.1016/S0092-8674(00)00136-7 . 16640087 . free .
• Habel JE, Ohren JF, Borgstahl GE . Dynamic light-scattering analysis of full-length human RPA14/32 dimer: purification, crystallization and self-association . Acta Crystallographica Section D . 57 . Pt 2 . 254–9 . Feb 2001 . 11173472 . 10.1107/S0907444900015225 . free .

Notes and References

1. Umbricht CB, Erdile LF, Jabs EW, Kelly TJ . Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A . The Journal of Biological Chemistry . 268 . 9 . 6131–8 . Mar 1993 . 10.1016/S0021-9258(18)53229-4 . 8454588 . free .
2. Web site: Entrez Gene: RPA2 Replication protein A2, 32kDa.
3. Otterlei M, Warbrick E, Nagelhus TA, Haug T, Slupphaug G, Akbari M, Aas PA, Steinsbekk K, Bakke O, Krokan HE . Post-replicative base excision repair in replication foci . The EMBO Journal . 18 . 13 . 3834–44 . Jul 1999 . 10393198 . 1171460 . 10.1093/emboj/18.13.3834 .
4. Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y . Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes . The EMBO Journal . 18 . 5 . 1397–406 . Mar 1999 . 10064605 . 1171229 . 10.1093/emboj/18.5.1397 .
5. Sukhodolets KE, Hickman AB, Agarwal SK, Sukhodolets MV, Obungu VH, Novotny EA, Crabtree JS, Chandrasekharappa SC, Collins FS, Spiegel AM, Burns AL, Marx SJ . The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene . Molecular and Cellular Biology . 23 . 2 . 493–509 . Jan 2003 . 12509449 . 151531 . 10.1128/mcb.23.2.493-509.2003.
6. Bochkareva E, Frappier L, Edwards AM, Bochkarev A . The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain . The Journal of Biological Chemistry . 273 . 7 . 3932–6 . Feb 1998 . 9461578 . 10.1074/jbc.273.7.3932. free .
7. Bochkareva E, Korolev S, Lees-Miller SP, Bochkarev A . Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA . The EMBO Journal . 21 . 7 . 1855–63 . Apr 2002 . 11927569 . 125950 . 10.1093/emboj/21.7.1855 .
8. Kim J, Kim D, Chung J . Replication protein a 32 kDa subunit (RPA p32) binds the SH2 domain of STAT3 and regulates its transcriptional activity . Cell Biology International . 24 . 7 . 467–73 . 10875894 . 10.1006/cbir.2000.0525 . 2000. 23783745 .
9. Yoo E, Kim BU, Lee SY, Cho CH, Chung JH, Lee CH . 53BP1 is associated with replication protein A and is required for RPA2 hyperphosphorylation following DNA damage . Oncogene . 24 . 35 . 5423–30 . Aug 2005 . 15856006 . 10.1038/sj.onc.1208710 . free .
10. Nagelhus TA, Haug T, Singh KK, Keshav KF, Skorpen F, Otterlei M, Bharati S, Lindmo T, Benichou S, Benarous R, Krokan HE . A sequence in the N-terminal region of human uracil-DNA glycosylase with homology to XPA interacts with the C-terminal part of the 34-kDa subunit of replication protein A . The Journal of Biological Chemistry . 272 . 10 . 6561–6 . Mar 1997 . 9045683 . 10.1074/jbc.272.10.6561. free .