40S ribosomal protein S8 explained

40S ribosomal protein S8 is a protein that in humans is encoded by the RPS8 gene.^{[1] [2]}

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal tumors and colon polyps compared to matched normal colonic mucosa has been observed. This gene is co-transcribed with the small nucleolar RNA genes U38A, U38B, U39, and U40, which are located in its fourth, fifth, first, and second introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.^[2]

Further reading

• Wool IG, Chan YL, Glück A . Structure and evolution of mammalian ribosomal proteins. . Biochem. Cell Biol. . 73 . 11–12 . 933–47 . 1996 . 8722009 . 10.1139/o95-101 .
• Pogue-Geile K, Geiser JR, Shu M, etal . Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein . Mol. Cell. Biol. . 11 . 8 . 3842–9 . 1991 . 1712897 . 10.1128/mcb.11.8.3842. 361167 .
• Vladimirov SN, Ivanov AV, Karpova GG, etal . Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry . Eur. J. Biochem. . 239 . 1 . 144–9 . 1996 . 8706699 . 10.1111/j.1432-1033.1996.0144u.x . free .
• Nicoloso M, Qu LH, Michot B, Bachellerie JP . Intron-encoded, antisense small nucleolar RNAs: the characterization of nine novel species points to their direct role as guides for the 2'-O-ribose methylation of rRNAs . J. Mol. Biol. . 260 . 2 . 178–95 . 1996 . 8764399 . 10.1006/jmbi.1996.0391 .
• Kenmochi N, Kawaguchi T, Rozen S, etal . A map of 75 human ribosomal protein genes . Genome Res. . 8 . 5 . 509–23 . 1998 . 9582194 . 10.1101/gr.8.5.509. free .
• Andersen JS, Lyon CE, Fox AH, etal . Directed proteomic analysis of the human nucleolus . Curr. Biol. . 12 . 1 . 1–11 . 2002 . 11790298 . 10.1016/S0960-9822(01)00650-9 . 14132033 . free . 2002CBio...12....1A .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Brenner C, Deplus R, Didelot C, etal . Myc represses transcription through recruitment of DNA methyltransferase corepressor . EMBO J. . 24 . 2 . 336–46 . 2005 . 15616584 . 10.1038/sj.emboj.7600509 . 545804 .
• Andersen JS, Lam YW, Leung AK, etal . Nucleolar proteome dynamics . Nature . 433 . 7021 . 77–83 . 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 .
• Yu Y, Ji H, Doudna JA, Leary JA . Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES-bound complexes . Protein Sci. . 14 . 6 . 1438–46 . 2005 . 15883184 . 10.1110/ps.041293005 . 2253395 .
• Tu LC, Yan X, Hood L, Lin B . Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells . Mol. Cell. Proteomics . 6 . 4 . 575–88 . 2007 . 17220478 . 10.1074/mcp.M600249-MCP200 . free .

Notes and References

1. Davies B, Fried M . The structure of the human intron-containing S8 ribosomal protein gene and determination of its chromosomal location at 1p32-p34.1 . Genomics . 15 . 1 . 68–75 . Mar 1993 . 8432552 . 10.1006/geno.1993.1011 .
2. Web site: Entrez Gene: RPS8 ribosomal protein S8.