40S ribosomal protein S29 explained

40S ribosomal protein S29 is a protein that in humans is encoded by the RPS29 gene.^{[1] [2] [3]}

Function

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit and a member of the S14P family of ribosomal proteins. The protein, which contains a C2-C2 zinc finger-like domain that can bind to zinc, can enhance the tumor suppressor activity of Ras-related protein 1A (KREV1). It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.^[3]

Clinical significance

Mutations in RPS29 cause Diamond–Blackfan anemia.^[4]

Further reading

• Wool IG, Chan YL, Glück A . Structure and evolution of mammalian ribosomal proteins . Biochemistry and Cell Biology . 73 . 11–12 . 933–47 . 1996 . 8722009 . 10.1139/o95-101 .
• Vladimirov SN, Ivanov AV, Karpova GG, Musolyamov AK, Egorov TA, Thiede B, Wittmann-Liebold B, Otto A . Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry . European Journal of Biochemistry . 239 . 1 . 144–9 . July 1996 . 8706699 . 10.1111/j.1432-1033.1996.0144u.x . free .
• Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC . A map of 75 human ribosomal protein genes . Genome Research . 8 . 5 . 509–23 . May 1998 . 9582194 . 10.1101/gr.8.5.509 . free .
• Coppock D, Kopman C, Gudas J, Cina-Poppe DA . Regulation of the quiescence-induced genes: quiescin Q6, decorin, and ribosomal protein S29 . Biochemical and Biophysical Research Communications . 269 . 2 . 604–10 . March 2000 . 10708601 . 10.1006/bbrc.2000.2324 .
• Bortoluzzi S, d'Alessi F, Romualdi C, Danieli GA . Differential expression of genes coding for ribosomal proteins in different human tissues . Bioinformatics . 17 . 12 . 1152–7 . December 2001 . 11751223 . 10.1093/bioinformatics/17.12.1152 . free .
• Yoshihama M, Uechi T, Asakawa S, Kawasaki K, Kato S, Higa S, Maeda N, Minoshima S, Tanaka T, Shimizu N, Kenmochi N . The human ribosomal protein genes: sequencing and comparative analysis of 73 genes . Genome Research . 12 . 3 . 379–90 . March 2002 . 11875025 . 155282 . 10.1101/gr.214202 .
• Zhou ZD, Bao L, Liu DG, Li MQ, Ge YZ, Huang YL, Liu WY . Low content of protein S29 in ribosomes of human lung cancer cell line a549: detected by two-dimensional electrophoresis . Protein and Peptide Letters . 10 . 1 . 91–7 . February 2003 . 12625830 . 10.2174/0929866033408273 .
• Yu Y, Ji H, Doudna JA, Leary JA . Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES-bound complexes . Protein Science . 14 . 6 . 1438–46 . June 2005 . 15883184 . 2253395 . 10.1110/ps.041293005 .
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . Large-scale mapping of human protein-protein interactions by mass spectrometry . Molecular Systems Biology . 3 . 1 . 89 . 2007 . 17353931 . 1847948 . 10.1038/msb4100134 .

Notes and References

1. Kondoh N, Noda M, Fisher RJ, Schweinfest CW, Papas TS, Kondoh A, Samuel KP, Oikawa T . The S29 ribosomal protein increases tumor suppressor activity of K rev-1 gene on v-K ras-transformed NIH3T3 cells . Biochimica et Biophysica Acta (BBA) - Molecular Cell Research . 1313 . 1 . 41–6 . August 1996 . 8781548 . 10.1016/0167-4889(96)00052-3 .
2. Frigerio JM, Dagorn JC, Iovanna JL . Cloning, sequencing and expression of the L5, L21, L27a, L28, S5, S9, S10 and S29 human ribosomal protein mRNAs . Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression . 1262 . 1 . 64–8 . May 1995 . 7772601 . 10.1016/0167-4781(95)00045-i .
3. Web site: Entrez Gene: RPS29 ribosomal protein S29.
4. Mirabello L, Macari ER, Jessop L, Ellis SR, Myers T, Giri N, Taylor AM, McGrath KE, Humphries JM, Ballew BJ, Yeager M, Boland JF, He J, Hicks BD, Burdett L, Alter BP, Zon L, Savage SA . Whole-exome sequencing and functional studies identify RPS29 as a novel gene mutated in multicase Diamond-Blackfan anemia families . Blood . 124 . 1 . 24–32 . July 2014 . 24829207 . 10.1182/blood-2013-11-540278 . 4125351 .