40S ribosomal protein S14 explained

40S ribosomal protein S14 is a protein that in humans is encoded by the RPS14 gene.^{[1] [2] [3]}

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor.^[3]

Further reading

• Wool IG, Chan YL, Glück A . Structure and evolution of mammalian ribosomal proteins. . Biochem. Cell Biol. . 73 . 11–12 . 933–47 . 1996 . 8722009 . 10.1139/o95-101 .
• Chen IT, Dixit A, Rhoads DD, Roufa DJ . Homologous ribosomal proteins in bacteria, yeast, and humans. . Proc. Natl. Acad. Sci. U.S.A. . 83 . 18 . 6907–11 . 1986 . 3529092 . 10.1073/pnas.83.18.6907 . 386619 . 1986PNAS...83.6907C . free .
• Dana S, Wasmuth JJ . Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5. . Mol. Cell. Biol. . 2 . 10 . 1220–8 . 1983 . 7177110 . 10.1128/mcb.2.10.1220. 369921 .
• Tasheva ES, Roufa DJ . Regulation of human RPS14 transcription by intronic antisense RNAs and ribosomal protein S14. . Genes Dev. . 9 . 3 . 304–16 . 1995 . 7867928 . 10.1101/gad.9.3.304 . free .
• Matoba R, Okubo K, Hori N, etal . The addition of 5'-coding information to a 3'-directed cDNA library improves analysis of gene expression. . Gene . 146 . 2 . 199–207 . 1994 . 8076819 . 10.1016/0378-1119(94)90293-3 .
• Vladimirov SN, Ivanov AV, Karpova GG, etal . Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry. . Eur. J. Biochem. . 239 . 1 . 144–9 . 1996 . 8706699 . 10.1111/j.1432-1033.1996.0144u.x . free .
• Martin-Nieto J, Roufa DJ . Functional analysis of human RPS14 null alleles. . J. Cell Sci. . 110 . 8. 955–63 . 1997 . 10.1242/jcs.110.8.955 . 9152021 . 10045/9720 . free .
• Kenmochi N, Kawaguchi T, Rozen S, etal . A map of 75 human ribosomal protein genes. . Genome Res. . 8 . 5 . 509–23 . 1998 . 9582194 . 10.1101/gr.8.5.509. free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Colland F, Jacq X, Trouplin V, etal . Functional proteomics mapping of a human signaling pathway. . Genome Res. . 14 . 7 . 1324–32 . 2004 . 15231748 . 10.1101/gr.2334104 . 442148 .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Yu Y, Ji H, Doudna JA, Leary JA . Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES-bound complexes. . Protein Sci. . 14 . 6 . 1438–46 . 2005 . 15883184 . 10.1110/ps.041293005 . 2253395 .
• Rual JF, Venkatesan K, Hao T, etal . Towards a proteome-scale map of the human protein-protein interaction network. . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Lim J, Hao T, Shaw C, etal . A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. . Cell . 125 . 4 . 801–14 . 2006 . 16713569 . 10.1016/j.cell.2006.03.032 . 13709685 . free .
• Ewing RM, Chu P, Elisma F, etal . Large-scale mapping of human protein-protein interactions by mass spectrometry. . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 .

Notes and References

1. Rhoads DD, Dixit A, Roufa DJ . Primary structure of human ribosomal protein S14 and the gene that encodes it . Mol Cell Biol . 6 . 8 . 2774–83 . Jan 1987 . 3785212 . 367844 . 10.1128/mcb.6.8.2774.
2. Diaz JJ, Roufa DJ . Fine-structure map of the human ribosomal protein gene RPS14 . Mol Cell Biol . 12 . 4 . 1680–6 . Apr 1992 . 1549121 . 369611 . 10.1128/mcb.12.4.1680.
3. Web site: Entrez Gene: RPS14 ribosomal protein S14.