RPN1 explained

Dolichyl-diphosphooligosaccharide—protein glycosyltransferase subunit 1 is an enzyme that in humans is encoded by the RPN1 gene.^[1]

This gene encodes a type I integral ribophorin membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein should not be confused with RPN1 of yeast, Drosophila, and C. elegans, which forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. The human version of this proteasome subunit is PSMD2.

Further reading

• Crimaudo C, Hortsch M, Gausepohl H, Meyer DI . Human ribophorins I and II: the primary structure and membrane topology of two highly conserved rough endoplasmic reticulum-specific glycoproteins. . EMBO J. . 6 . 1 . 75–82 . 1987 . 3034581 . 10.1002/j.1460-2075.1987.tb04721.x. 553359 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Pekarsky Y, Rynditch A, Wieser R, etal . Activation of a novel gene in 3q21 and identification of intergenic fusion transcripts with ecotropic viral insertion site I in leukemia. . Cancer Res. . 57 . 18 . 3914–9 . 1997 . 9307271 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Fu J, Kreibich G . Retention of subunits of the oligosaccharyltransferase complex in the endoplasmic reticulum. . J. Biol. Chem. . 275 . 6 . 3984–90 . 2000 . 10660554 . 10.1074/jbc.275.6.3984 . free .
• Dias Neto E, Correa RG, Verjovski-Almeida S, etal . Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. . Proc. Natl. Acad. Sci. U.S.A. . 97 . 7 . 3491–6 . 2000 . 10737800 . 10.1073/pnas.97.7.3491 . 16267 . 2000PNAS...97.3491D . free .
• Elsasser S, Gali RR, Schwickart M, etal . Proteasome subunit Rpn1 binds ubiquitin-like protein domains. . Nat. Cell Biol. . 4 . 9 . 725–30 . 2002 . 12198498 . 10.1038/ncb845 . 25666734 .
• Saeki Y, Sone T, Toh-e A, Yokosawa H . Identification of ubiquitin-like protein-binding subunits of the 26S proteasome. . Biochem. Biophys. Res. Commun. . 296 . 4 . 813–9 . 2002 . 12200120 . 10.1016/S0006-291X(02)02002-8 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Basrur V, Yang F, Kushimoto T, etal . Proteomic analysis of early melanosomes: identification of novel melanosomal proteins. . J. Proteome Res. . 2 . 1 . 69–79 . 2003 . 12643545 . 10.1021/pr025562r .
• Kelleher DJ, Karaoglu D, Mandon EC, Gilmore R . Oligosaccharyltransferase isoforms that contain different catalytic STT3 subunits have distinct enzymatic properties. . Mol. Cell . 12 . 1 . 101–11 . 2003 . 12887896 . 10.1016/S1097-2765(03)00243-0 . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Shibatani T, David LL, McCormack AL, etal . Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits. . Biochemistry . 44 . 16 . 5982–92 . 2005 . 15835887 . 10.1021/bi047328f .
• Rual JF, Venkatesan K, Hao T, etal . Towards a proteome-scale map of the human protein-protein interaction network. . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Lim J, Hao T, Shaw C, etal . A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. . Cell . 125 . 4 . 801–14 . 2006 . 16713569 . 10.1016/j.cell.2006.03.032 . 13709685 . free .
• Chi A, Valencia JC, Hu ZZ, etal . Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes. . J. Proteome Res. . 5 . 11 . 3135–44 . 2007 . 17081065 . 10.1021/pr060363j .
• Ewing RM, Chu P, Elisma F, etal . Large-scale mapping of human protein-protein interactions by mass spectrometry. . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 .

Notes and References

1. Web site: Entrez Gene: RPN1 ribophorin I.