60S acidic ribosomal protein P0 explained

60S acidic ribosomal protein P0 is a protein that in humans is encoded by the RPLP0 gene.^{[1] [2]}

Ribosomes catalyze protein synthesis and consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.^[2]

Further reading

• Wool IG, Chan YL, Glück A . Structure and evolution of mammalian ribosomal proteins. . Biochem. Cell Biol. . 73 . 11–12 . 933–47 . 1996 . 8722009 . 10.1139/o95-101 .
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• Rich BE, Steitz JA . Human acidic ribosomal phosphoproteins P0, P1, and P2: analysis of cDNA clones, in vitro synthesis, and assembly . Mol. Cell. Biol. . 7 . 11 . 4065–74 . 1988 . 3323886 . 10.1128/mcb.7.11.4065. 368077 .
• Towbin H . Monoclonal antibodies against eucaryotic ribosomes. Use to characterize a ribosomal protein not previously identified and antigenically related to the acidic phosphoproteins P1/P2 . J. Biol. Chem. . 257 . 21 . 12709–15 . 1982 . 6182142 . vanc. Ramjoué HP . Kuster H . 3 . Liverani . D . Gordon . J . 10.1016/S0021-9258(18)33569-5 . free .
• Kato S . Construction of a human full-length cDNA bank . Gene . 150 . 2 . 243–50 . 1995 . 7821789 . 10.1016/0378-1119(94)90433-2 . vanc. Sekine S . Oh SW . 3 . Nam-Soon . Kim . Yuri . Umezawa . Naoto . Abe . Midori . Yokoyama-Kobayashi . Takashi . Aoki .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 . vanc. Yoshitomo-Nakagawa K . Maruyama K . 3 . Suyama . Akira . Sugano . Sumio .
• Toward a complete human genome sequence . Genome Res. . 8 . 11 . 1097–108 . 1999 . 9847074 . 10.1101/gr.8.11.1097 . . free .
• Tchórzewski M, Boldyreff B, Issinger O, Grankowski N . Analysis of the protein-protein interactions between the human acidic ribosomal P-proteins: evaluation by the two hybrid system . Int. J. Biochem. Cell Biol. . 32 . 7 . 737–46 . 2000. 10856704 . 10.1016/S1357-2725(00)00017-0 .
• Chan SH, Hung FS, Chan DS, Shaw PC . Trichosanthin interacts with acidic ribosomal proteins P0 and P1 and mitotic checkpoint protein MAD2B . Eur. J. Biochem. . 268 . 7 . 2107–12 . 2001 . 11277934 . 10.1046/j.1432-1327.2001.02091.x . free .
• Uechi T, Tanaka T, Kenmochi N . A complete map of the human ribosomal protein genes: assignment of 80 genes to the cytogenetic map and implications for human disorders . Genomics . 72 . 3 . 223–30 . 2001 . 11401437 . 10.1006/geno.2000.6470 .
• Andersen JS . Directed proteomic analysis of the human nucleolus . Curr. Biol. . 12 . 1 . 1–11 . 2002 . 11790298 . 10.1016/S0960-9822(01)00650-9 . vanc. Lyon CE . Fox AH . 3 . Leung . Anthony K.L. . Lam . Yun Wah . Steen . Hanno . Mann . Matthias . Lamond . Angus I. . 14132033 . free . 2002CBio...12....1A .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . vanc. Feingold EA . Grouse LH . 3 . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M . free .
• Tchórzewski M . The subcellular distribution of the human ribosomal "stalk" components: P1, P2 and P0 proteins . Int. J. Biochem. Cell Biol. . 35 . 2 . 203–11 . 2003 . 12479870 . 10.1016/S1357-2725(02)00133-4 . vanc. Krokowski D . Rzeski W . 3 . Issinger . Olaf-Georg . Grankowski . Nikodem .
• Ota T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . vanc. Suzuki Y . Nishikawa T . 3 . Otsuki . Tetsuji . Sugiyama . Tomoyasu . Irie . Ryotaro . Wakamatsu . Ai . Hayashi . Koji . Sato . Hiroyuki . free .
• Shu H . Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line . Mol. Cell. Proteomics . 3 . 3 . 279–86 . 2004 . 14729942 . 10.1074/mcp.D300003-MCP200 . vanc. Chen S . Bi Q . 3 . Mumby . M . Brekken . DL . free .
• Bouwmeester T . A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway . Nat. Cell Biol. . 6 . 2 . 97–105 . 2004 . 14743216 . 10.1038/ncb1086 . vanc. Bauch A . Ruffner H . 3 . Angrand . Pierre-Olivier . Bergamini . Giovanna . Croughton . Karen . Cruciat . Cristina . Eberhard . Dirk . Gagneur . Julien . 11683986 .
• Villacé P, Marión RM, Ortín J . The composition of Staufen-containing RNA granules from human cells indicates their role in the regulated transport and translation of messenger RNAs . Nucleic Acids Res. . 32 . 8 . 2411–20 . 2004 . 15121898 . 10.1093/nar/gkh552 . 419443 .

Notes and References

1. Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC . A map of 75 human ribosomal protein genes . Genome Res . 8 . 5 . 509–23 . Aug 1998 . 9582194 . 10.1101/gr.8.5.509. free .
2. Web site: Entrez Gene: RPLP0 ribosomal protein, large, P0.