60S ribosomal protein L8 explained

60S ribosomal protein L8 is a protein that in humans is encoded by the RPL8 gene.^{[1] [2] [3]}

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.^[3]

Further reading

• Wool IG, Chan YL, Glück A . Structure and evolution of mammalian ribosomal proteins. . Biochem. Cell Biol. . 73 . 11–12 . 933–47 . 1996 . 8722009 . 10.1139/o95-101 .
• Uhlein M, Weglöhner W, Urlaub H, Wittmann-Liebold B . Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies . Biochem. J. . 331 . 2. 423–30 . 1998 . 9531480 . 10.1042/bj3310423. 1219371 .
• Andersen JS, Lyon CE, Fox AH, etal . Directed proteomic analysis of the human nucleolus . Curr. Biol. . 12 . 1 . 1–11 . 2002 . 11790298 . 10.1016/S0960-9822(01)00650-9 . 14132033 . free . 2002CBio...12....1A .
• Yoshihama M, Uechi T, Asakawa S, etal . The human ribosomal protein genes: sequencing and comparative analysis of 73 genes . Genome Res. . 12 . 3 . 379–90 . 2002 . 11875025 . 10.1101/gr.214202 . 155282 .
• Brezillon S, Lannoy V, Franssen JD, etal . Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8 . J. Biol. Chem. . 278 . 2 . 776–83 . 2003 . 12401809 . 10.1074/jbc.M206396200 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Odintsova TI, Müller EC, Ivanov AV, etal . Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing . J. Protein Chem. . 22 . 3 . 249–58 . 2004 . 12962325 . 10.1023/A:1025068419698 . 10710245 .
• Bouwmeester T, Bauch A, Ruffner H, etal . A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway . Nat. Cell Biol. . 6 . 2 . 97–105 . 2004 . 14743216 . 10.1038/ncb1086 . 11683986 .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Rush J, Moritz A, Lee KA, etal . Immunoaffinity profiling of tyrosine phosphorylation in cancer cells . Nat. Biotechnol. . 23 . 1 . 94–101 . 2005 . 15592455 . 10.1038/nbt1046 . 7200157 .
• Andersen JS, Lam YW, Leung AK, etal . Nucleolar proteome dynamics . Nature . 433 . 7021 . 77–83 . 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 .
• Stelzl U, Worm U, Lalowski M, etal . A human protein-protein interaction network: a resource for annotating the proteome . Cell . 122 . 6 . 957–68 . 2005 . 16169070 . 10.1016/j.cell.2005.08.029 . 11858/00-001M-0000-0010-8592-0 . 8235923 . free .

Notes and References

1. Hanes J, Klaudiny J, von der Kammer H, Scheit KH . Characterization by cDNA cloning of the mRNA of human ribosomal protein L8 . Biochem Biophys Res Commun . 197 . 3 . 1223–8 . Feb 1994 . 7506540 . 10.1006/bbrc.1993.2607 .
2. Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC . A map of 75 human ribosomal protein genes . Genome Res . 8 . 5 . 509–23 . Aug 1998 . 9582194 . 10.1101/gr.8.5.509. free .
3. Web site: Entrez Gene: RPL8 ribosomal protein L8.