60S ribosomal protein L14 explained

60S ribosomal protein L14 is a protein that in humans is encoded by the RPL14 gene.^{[1] [2]}

Function

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.^[2]

Interactions

RPL14 has been shown to interact with PHLDA1.^[3]

Further reading

• Wool IG, Chan YL, Glück A . Structure and evolution of mammalian ribosomal proteins. . Biochem. Cell Biol. . 73 . 11–12 . 933–47 . 1996 . 8722009 . 10.1139/o95-101 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Dave VP, Larché M, Rencher SD, Koop BF, Hurwitz JL . Restricted usage of T-cell receptor V alpha sequence and variable-joining pairs after normal T-cell development and bone marrow transplantation. . Hum. Immunol. . 37 . 3 . 178–84 . 1994 . 8244780 . 10.1016/0198-8859(93)90183-2 .
• Li SH, McInnis MG, Margolis RL, Antonarakis SE, Ross CA . Novel triplet repeat containing genes in human brain: cloning, expression, and length polymorphisms. . Genomics . 16 . 3 . 572–9 . 1993 . 8325628 . 10.1006/geno.1993.1232 . free .
• Aoki M, Koranyi L, Riggs AC, Wasson J, Chiu KC, Vaxillaire M, Froguel P, Gough S, Liu L, Donis-Keller H . Identification of trinucleotide repeat-containing genes in human pancreatic islets. . Diabetes . 45 . 2 . 157–64 . 1996 . 8549859 . 10.2337/diabetes.45.2.157 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Reddy PH, Stockburger E, Gillevet P, Tagle DA . Mapping and characterization of novel (CAG)n repeat cDNAs from adult human brain derived by the oligo capture method. . Genomics . 46 . 2 . 174–82 . 1998 . 9417904 . 10.1006/geno.1997.5044 .
• Hinz T, Flindt S, Marx A, Janssen O, Kabelitz D . Inhibition of protein synthesis by the T cell receptor-inducible human TDAG51 gene product. . Cell. Signal. . 13 . 5 . 345–52 . 2001 . 11369516 . 10.1016/S0898-6568(01)00141-3 .
• Uechi T, Tanaka T, Kenmochi N . A complete map of the human ribosomal protein genes: assignment of 80 genes to the cytogenetic map and implications for human disorders. . Genomics . 72 . 3 . 223–30 . 2001 . 11401437 . 10.1006/geno.2000.6470 .
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI . Directed proteomic analysis of the human nucleolus. . Curr. Biol. . 12 . 1 . 1–11 . 2002 . 11790298 . 10.1016/S0960-9822(01)00650-9 . 14132033 . free . 2002CBio...12....1A .
• Yoshihama M, Uechi T, Asakawa S, Kawasaki K, Kato S, Higa S, Maeda N, Minoshima S, Tanaka T, Shimizu N, Kenmochi N . The human ribosomal protein genes: sequencing and comparative analysis of 73 genes. . Genome Res. . 12 . 3 . 379–90 . 2002 . 11875025 . 155282 . 10.1101/gr.214202 .
• Odintsova TI, Müller EC, Ivanov AV, Egorov TA, Bienert R, Vladimirov SN, Kostka S, Otto A, Wittmann-Liebold B, Karpova GG . Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing. . J. Protein Chem. . 22 . 3 . 249–58 . 2004 . 12962325 . 10.1023/A:1025068419698 . 10710245 .
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M . Nucleolar proteome dynamics. . Nature . 433 . 7021 . 77–83 . 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 .
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . 7827573 . free .

Notes and References

1. Tanaka M, Tanaka T, Harata M, Suzuki T, Mitsui Y . Triplet repeat-containing ribosomal protein L14 gene in immortalized human endothelial cell line (t-HUE4) . Biochem Biophys Res Commun . 243 . 2 . 531–7 . March 1998 . 9480843 . 10.1006/bbrc.1998.8125 .
2. Web site: Entrez Gene: RPL14 ribosomal protein L14.
3. Hinz T, Flindt S, Marx A, Janssen O, Kabelitz D . Inhibition of protein synthesis by the T cell receptor-inducible human TDAG51 gene product . Cell. Signal. . 13 . 5 . 345–52 . May 2001 . 11369516 . 10.1016/S0898-6568(01)00141-3 .