RPH3A explained

Rabphilin-3A is a protein that in humans is encoded by the RPH3A gene.^{[1] [2] [3]} It contains two C2 domains and binds calcium ions at low micromolar concentration. Rabphilin was shown to regulate neurotransmitter release in hippocampal neurons after neurons had an increased synaptic activity and their release rate was depressed.^[4]

Interactions

RPH3A has been shown to interact with RAB3A,^{[5] [6] [7]} RAB3B^{[5] [7]} and CASK.^[8]

Further reading

• Miyazaki M, Kaibuchi K, Shirataki H, etal . Rabphilin-3A binds to a M(r) 115,000 polypeptide in a phosphatidylserine- and Ca(2+)-dependent manner. . Brain Res. Mol. Brain Res. . 28 . 1 . 29–36 . 1995 . 7707875 . 10.1016/0169-328X(94)00180-M .
• Imazumi K, Sasaki T, Takahashi K, Takai Y . Identification of a rabphilin-3A-interacting protein as GTP cyclohydrolase I in PC12 cells. . Biochem. Biophys. Res. Commun. . 205 . 2 . 1409–16 . 1995 . 7802677 . 10.1006/bbrc.1994.2822 .
• Miyazaki M, Shirataki H, Kohno H, etal . Identification as beta-adducin of a protein interacting with rabphilin-3A in the presence of Ca2+ and phosphatidylserine. . Biochem. Biophys. Res. Commun. . 205 . 1 . 460–6 . 1995 . 7999065 . 10.1006/bbrc.1994.2688 .
• Mizoguchi A, Yano Y, Hamaguchi H, etal . Localization of Rabphilin-3A on the synaptic vesicle. . Biochem. Biophys. Res. Commun. . 202 . 3 . 1235–43 . 1994 . 8060298 . 10.1006/bbrc.1994.2063 .
• Weber E, Jilling T, Kirk KL . Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. . J. Biol. Chem. . 271 . 12 . 6963–71 . 1996 . 8636125 . 10.1074/jbc.271.12.6963 . free .
• Perin MS . Mirror image motifs mediate the interaction of the COOH terminus of multiple synaptotagmins with the neurexins and calmodulin. . Biochemistry . 35 . 43 . 13808–16 . 1996 . 8901523 . 10.1021/bi960853x .
• Gilligan DM, Lozovatsky L, Silberfein A . Organization of the human beta-adducin gene (ADD2). . Genomics . 43 . 2 . 141–8 . 1997 . 9244430 . 10.1006/geno.1997.4802 . free .
• Ostermeier C, Brunger AT . Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A. . Cell . 96 . 3 . 363–74 . 1999 . 10025402 . 10.1016/S0092-8674(00)80549-8 . 15162326 . free .
• Zhang Y, Luan Z, Liu A, Hu G . The scaffolding protein CASK mediates the interaction between rabphilin3a and beta-neurexins. . FEBS Lett. . 497 . 2–3 . 99–102 . 2001 . 11377421 . 10.1016/S0014-5793(01)02450-4 . 33119468 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Fukuda M . Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. . J. Biol. Chem. . 278 . 17 . 15373–80 . 2003 . 12578829 . 10.1074/jbc.M212341200 . free .
• Sun L, Bittner MA, Holz RW . Rim, a component of the presynaptic active zone and modulator of exocytosis, binds 14-3-3 through its N terminus. . J. Biol. Chem. . 278 . 40 . 38301–9 . 2003 . 12871946 . 10.1074/jbc.M212801200 . free .
• Rastaldi MP, Armelloni S, Berra S, etal . Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a. . Am. J. Pathol. . 163 . 3 . 889–99 . 2003 . 12937130 . 10.1016/S0002-9440(10)63449-9. 1868247 .
• Dalfó E, Barrachina M, Rosa JL, etal . Abnormal alpha-synuclein interactions with rab3a and rabphilin in diffuse Lewy body disease. . Neurobiol. Dis. . 16 . 1 . 92–7 . 2004 . 15207266 . 10.1016/j.nbd.2004.01.001 . 5793677 .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Smith R, Klein P, Koc-Schmitz Y, etal . Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease. . J. Neurochem. . 103 . 1 . 115–23 . 2007 . 17877635 . 10.1111/j.1471-4159.2007.04703.x . free .
• Katkoori VR, Jia X, Chatla C, etal . Clinical significance of a novel single nucleotide polymorphism in the 5' untranslated region of the Rabphillin-3A-Like gene in colorectal adenocarcinoma. . Front. Biosci. . 13 . 13. 1050–61 . 2007 . 17981610 . 10.2741/2742 . 2667692 .

Notes and References

1. Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O . Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro . DNA Res . 6 . 1 . 63–70 . July 1999 . 10231032 . 10.1093/dnares/6.1.63 . free .
2. Inagaki N, Mizuta M, Seino S . Cloning of a mouse Rabphilin-3A expressed in hormone-secreting cells . J Biochem . 116 . 2 . 239–42 . February 1995 . 7822236 . 10.1093/oxfordjournals.jbchem.a124512.
3. Web site: Entrez Gene: RPH3A rabphilin 3A homolog (mouse).
4. Deák F, Shin OH, Tang J, Hanson P, Ubach J, Jahn R, Rizo J, Kavalali ET, Südhof TC . Rabphilin regulates SNARE-dependent re-priming of synaptic vesicles for fusion. . EMBO J. . 25 . 12 . 2856–66 . 2006 . 16763567 . 10.1038/sj.emboj.7601165 . 1500841 .
5. Fukuda . Mitsunori . April 2003 . Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2 . J. Biol. Chem. . 278 . 17 . 15373–80 . 0021-9258. 12578829 . 10.1074/jbc.M212341200 . free .
6. 10.1016/S0092-8674(00)80549-8 . Ostermeier . C . Brunger A T . February 1999 . Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A . Cell . 96 . 3 . 363–74 . 0092-8674. 10025402 . 15162326 . free .
7. 10.1074/jbc.271.12.6963 . Weber . E . Jilling T . Kirk K L . March 1996 . Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells . J. Biol. Chem. . 271 . 12 . 6963–71 . 0021-9258. 8636125 . free .
8. 10.1016/S0014-5793(01)02450-4 . Zhang . Y . Luan Z . Liu A . Hu G . May 2001 . The scaffolding protein CASK mediates the interaction between rabphilin3a and beta-neurexins . FEBS Lett. . 497 . 2–3 . 99–102 . 0014-5793. 11377421 . 33119468 . free .