RNF7 explained

RING-box protein 2 is a protein that in humans is encoded by the RNF7 gene.^{[1] [2] [3]}

The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Interactions

RNF7 has been shown to interact with CSNK2B.^[4]

See also

• RING finger domain

Further reading

• Tan P, Fuchs SY, Chen A, etal . Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha . Mol. Cell . 3 . 4 . 527–33 . 1999 . 10230406 . 10.1016/S1097-2765(00)80481-5 . free .
• Swaroop M, Bian J, Aviram M, etal . Expression, purification, and biochemical characterization of SAG, a ring finger redox-sensitive protein . Free Radic. Biol. Med. . 27 . 1–2 . 193–202 . 1999 . 10443936 . 10.1016/S0891-5849(99)00078-7 .
• Sun Y . Alterations of SAG mRNA in human cancer cell lines: requirement for the RING finger domain for apoptosis protection . Carcinogenesis . 20 . 10 . 1899–903 . 1999 . 10506102 . 10.1093/carcin/20.10.1899 . free .
• Son MY, Park JW, Kim YS, etal . Protein kinase CKII interacts with and phosphorylates the SAG protein containing ring-H2 finger motif . Biochem. Biophys. Res. Commun. . 263 . 3 . 743–8 . 1999 . 10512750 . 10.1006/bbrc.1999.1460 .
• Chen A, Wu K, Fuchs SY, etal . The conserved RING-H2 finger of ROC1 is required for ubiquitin ligation . J. Biol. Chem. . 275 . 20 . 15432–9 . 2000 . 10748083 . 10.1074/jbc.M907300199 . free .
• Swaroop M, Wang Y, Miller P, etal . Yeast homolog of human SAG/ROC2/Rbx2/Hrt2 is essential for cell growth, but not for germination: chip profiling implicates its role in cell cycle regulation . Oncogene . 19 . 24 . 2855–66 . 2000 . 10851089 . 10.1038/sj.onc.1203635 . free .
• Furukawa M, Zhang Y, McCarville J, etal . The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1 . Mol. Cell. Biol. . 20 . 21 . 8185–97 . 2000 . 11027288 . 10.1128/MCB.20.21.8185-8197.2000 . 86428 .
• Duan H, Tsvetkov LM, Liu Y, etal . Promotion of S-phase entry and cell growth under serum starvation by SAG/ROC2/Rbx2/Hrt2, an E3 ubiquitin ligase component: association with inhibition of p27 accumulation . Mol. Carcinog. . 30 . 1 . 37–46 . 2001 . 11255262 . 10.1002/1098-2744(200101)30:1<37::AID-MC1011>3.0.CO;2-7 . 12999764 .
• Swaroop M, Gosink M, Sun Y . SAG/ROC2/Rbx2/Hrt2, a component of SCF E3 ubiquitin ligase: genomic structure, a splicing variant, and two family pseudogenes . DNA Cell Biol. . 20 . 7 . 425–34 . 2001 . 11506706 . 10.1089/104454901750361488 . 2027.42/63441 . free .
• Ahn BH, Kim TH, Bae YS . Mapping of the interaction domain of the protein kinase CKII beta subunit with target proteins . Mol. Cells . 12 . 2 . 158–63 . 2002 . 10.1016/S1016-8478(23)17077-4 . 11710515 . free .
• Furukawa M, Ohta T, Xiong Y . Activation of UBC5 ubiquitin-conjugating enzyme by the RING finger of ROC1 and assembly of active ubiquitin ligases by all cullins . J. Biol. Chem. . 277 . 18 . 15758–65 . 2002 . 11861641 . 10.1074/jbc.M108565200 . free .
• Kim SY, Bae YS, Park JW . Thiol-linked peroxidase activity of human sensitive to apoptosis gene (SAG) protein . Free Radic. Res. . 36 . 1 . 73–8 . 2002 . 11999705 . 10.1080/10715760210164 . 39810540 .
• Kim YS, Ha KS, Kim YH, Bae YS . The Ring-H2 finger motif of CKBBP1/SAG is necessary for interaction with protein kinase CKII and optimal cell proliferation . J. Biochem. Mol. Biol. . 35 . 6 . 629–36 . 2003 . 12470599 . 10.5483/bmbrep.2002.35.6.629. free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Kim SY, Lee JH, Yang ES, etal . Human sensitive to apoptosis gene protein inhibits peroxynitrite-induced DNA damage . Biochem. Biophys. Res. Commun. . 301 . 3 . 671–4 . 2003 . 12565832 . 10.1016/S0006-291X(03)00018-4 .
• Kim YS, Lee JY, Son MY, etal . Phosphorylation of threonine 10 on CKBBP1/SAG/ROC2/Rbx2 by protein kinase CKII promotes the degradation of IkappaBalpha and p27Kip1 . J. Biol. Chem. . 278 . 31 . 28462–9 . 2003 . 12748192 . 10.1074/jbc.M302584200 . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Rual JF, Venkatesan K, Hao T, etal . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .

Notes and References

1. Duan H, Wang Y, Aviram M, Swaroop M, Loo JA, Bian J, Tian Y, Mueller T, Bisgaier CL, Sun Y . SAG, a novel zinc RING finger protein that protects cells from apoptosis induced by redox agents . Mol Cell Biol . 19 . 4 . 3145–55 . April 1999 . 10082581 . 84108 . 10.1128/MCB.19.4.3145.
2. Ohta T, Michel JJ, Schottelius AJ, Xiong Y . ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity . Mol Cell . 3 . 4 . 535–41 . May 1999 . 10230407 . 10.1016/S1097-2765(00)80482-7 . 19371828 . free .
3. Web site: Entrez Gene: RNF7 ring finger protein 7.
4. Ahn . B H . Kim T H . Bae Y S . October 2001 . Mapping of the interaction domain of the protein kinase CKII beta subunit with target proteins . Mol. Cells . 12 . 2 . 158–63 . Korea (South). 10.1016/S1016-8478(23)17077-4 . 1016-8478. 11710515 . free .