RNF216 explained

E3 ubiquitin-protein ligase RNF216 is an enzyme that in humans is encoded by the RNF216 gene.^[1]

This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.

See also

• RING finger domain

Interactions

RNF216 has been shown to interact with TLR9^[2] and RIPK1.^[3]

Further reading

• Lake JA, Carr J, Feng F, etal . The role of Vif during HIV-1 infection: interaction with novel host cellular factors . J. Clin. Virol. . 26 . 2 . 143–52 . 2003 . 12600646 . 10.1016/S1386-6532(02)00113-0 .
• Matoba R, Okubo K, Hori N, etal . The addition of 5'-coding information to a 3'-directed cDNA library improves analysis of gene expression . Gene . 146 . 2 . 199–207 . 1994 . 8076819 . 10.1016/0378-1119(94)90293-3 .
• van der Reijden BA, Erpelinck-Verschueren CA, Löwenberg B, Jansen JH . TRIADs: a new class of proteins with a novel cysteine-rich signature . Protein Sci. . 8 . 7 . 1557–61 . 1999 . 10422847 . 10.1110/ps.8.7.1557 . 2144383 .
• Wang X, Stollar BD . Immunoglobulin VH gene expression in human aging . Clin. Immunol. . 93 . 2 . 132–42 . 1999 . 10527689 . 10.1006/clim.1999.4781 .
• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination . Genome Res. . 10 . 11 . 1788–95 . 2001 . 11076863 . 10.1101/gr.143000 . 310948 .
• Wiemann S, Weil B, Wellenreuther R, etal . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Res. . 11 . 3 . 422–35 . 2001 . 11230166 . 10.1101/gr.GR1547R . 311072 .
• Simpson JC, Wellenreuther R, Poustka A, etal . Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing . EMBO Rep. . 1 . 3 . 287–92 . 2001 . 11256614 . 10.1093/embo-reports/kvd058 . 1083732 .
• Chen D, Li X, Zhai Z, Shu HB . A novel zinc finger protein interacts with receptor-interacting protein (RIP) and inhibits tumor necrosis factor (TNF)- and IL1-induced NF-kappa B activation . J. Biol. Chem. . 277 . 18 . 15985–91 . 2002 . 11854271 . 10.1074/jbc.M108675200 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Hillier LW, Fulton RS, Fulton LA, etal . The DNA sequence of human chromosome 7 . Nature . 424 . 6945 . 157–64 . 2003 . 12853948 . 10.1038/nature01782 . 2003Natur.424..157H . free .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Chuang TH, Ulevitch RJ . Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors . Nat. Immunol. . 5 . 5 . 495–502 . 2004 . 15107846 . 10.1038/ni1066 . 39773935 .
• Feng F, Davis A, Lake JA, etal . Ring finger protein ZIN interacts with human immunodeficiency virus type 1 Vif . J. Virol. . 78 . 19 . 10574–81 . 2004 . 15367624 . 10.1128/JVI.78.19.10574-10581.2004 . 516435 .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Wiemann S, Arlt D, Huber W, etal . From ORFeome to biology: a functional genomics pipeline . Genome Res. . 14 . 10B . 2136–44 . 2004 . 15489336 . 10.1101/gr.2576704 . 528930 .
• Oh JH, Yang JO, Hahn Y, etal . Transcriptome analysis of human gastric cancer . Mamm. Genome . 16 . 12 . 942–54 . 2006 . 16341674 . 10.1007/s00335-005-0075-2 . 69278 .
• Mehrle A, Rosenfelder H, Schupp I, etal . The LIFEdb database in 2006 . Nucleic Acids Res. . 34 . Database issue . D415–8 . 2006 . 16381901 . 10.1093/nar/gkj139 . 1347501 .
• Fearns C, Pan Q, Mathison JC, Chuang TH . Triad3A regulates ubiquitination and proteasomal degradation of RIP1 following disruption of Hsp90 binding . J. Biol. Chem. . 281 . 45 . 34592–600 . 2006 . 16968706 . 10.1074/jbc.M604019200 . free .

Notes and References

1. Web site: Entrez Gene: TRIAD3 TRIAD3 protein.
2. Chuang . Tsung-Hsien . Ulevitch Richard J . May 2004 . Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors . Nat. Immunol. . 5 . 5 . 495–502 . 1529-2908. 15107846 . 10.1038/ni1066 . 39773935 .
3. Chen . Danying . Li Xiaoyan . Zhai Zhonghe . . May 2002 . A novel zinc finger protein interacts with receptor-interacting protein (RIP) and inhibits tumor necrosis factor (TNF)- and IL1-induced NF-kappa B activation . J. Biol. Chem. . 277 . 18 . 15985–91 . 0021-9258. 11854271 . 10.1074/jbc.M108675200 . free .