RNASEH1 explained

Ribonuclease H1 also known as RNase H1 is an enzyme that in humans is encoded by the RNASEH1 gene.^{[1] [2] [3]} The RNase H1 is a non-specific endonuclease and catalyzes the cleavage of RNA via a hydrolytic mechanism.

Further reading

• Nowotny M, Cerritelli SM, Ghirlando R, etal . Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD. . EMBO J. . 27 . 7 . 1172–81 . 2008 . 18337749 . 10.1038/emboj.2008.44 . 2323259 .
• Lima WF, Wu H, Nichols JG, etal . Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate. . J. Biol. Chem. . 278 . 50 . 49860–7 . 2003 . 14506260 . 10.1074/jbc.M306543200 . free .
• Lima WF, Rose JB, Nichols JG, etal . The positional influence of the helical geometry of the heteroduplex substrate on human RNase H1 catalysis. . Mol. Pharmacol. . 71 . 1 . 73–82 . 2007 . 17028157 . 10.1124/mol.106.025429 . 27765676 .
• Lima WF, Nichols JG, Wu H, etal . Structural requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis. . J. Biol. Chem. . 279 . 35 . 36317–26 . 2004 . 15205459 . 10.1074/jbc.M405035200 . free .
• Otsuki T, Ota T, Nishikawa T, etal . Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. . DNA Res. . 12 . 2 . 117–26 . 2005 . 16303743 . 10.1093/dnares/12.2.117 . free .
• Wu H, Lima WF, Crooke ST . Properties of cloned and expressed human RNase H1. . J. Biol. Chem. . 274 . 40 . 28270–8 . 1999 . 10497183 . 10.1074/jbc.274.40.28270 . free .
• Frank P, Braunshofer-Reiter C, Pöltl A, Holzmann K . Cloning, subcellular localization and functional expression of human RNase HII. . Biol. Chem. . 379 . 12 . 1407–12 . 1998 . 9894807 . 10.1515/bchm.1998.379.12.1407 . 19692186 .
• Wu H, Lima WF, Zhang H, etal . Determination of the role of the human RNase H1 in the pharmacology of DNA-like antisense drugs. . J. Biol. Chem. . 279 . 17 . 17181–9 . 2004 . 14960586 . 10.1074/jbc.M311683200 . free .
• Hillier LW, Graves TA, Fulton RS, etal . Generation and annotation of the DNA sequences of human chromosomes 2 and 4. . Nature . 434 . 7034 . 724–31 . 2005 . 15815621 . 10.1038/nature03466 . 2005Natur.434..724H . free .
• Lima WF, Wu H, Nichols JG, etal . Human RNase H1 activity is regulated by a unique redox switch formed between adjacent cysteines. . J. Biol. Chem. . 278 . 17 . 14906–12 . 2003 . 12473655 . 10.1074/jbc.M211279200 . free .
• ten Asbroek AL, van Groenigen M, Nooij M, Baas F . The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides. . Eur. J. Biochem. . 269 . 2 . 583–92 . 2002 . 11856317 . 10.1046/j.0014-2956.2001.02686.x . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Cerritelli SM, Crouch RJ . Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI. . Genomics . 53 . 3 . 300–7 . 1998 . 9799596 . 10.1006/geno.1998.5497 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2002 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Cerritelli SM, Frolova EG, Feng C, etal . Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice. . Mol. Cell . 11 . 3 . 807–15 . 2003 . 12667461 . 10.1016/S1097-2765(03)00088-1 . free .
• Lima WF, Rose JB, Nichols JG, etal . Human RNase H1 discriminates between subtle variations in the structure of the heteroduplex substrate. . Mol. Pharmacol. . 71 . 1 . 83–91 . 2007 . 17028158 . 10.1124/mol.106.025015 . 24995048 .

Notes and References

1. Web site: Entrez Gene: ribonuclease H1.
2. ten Asbroek AL, van Groenigen M, Jakobs ME, Koevoets C, Janssen B, Baas F . Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome . Genomics . 79 . 6 . 818–23 . June 2002 . 12036296 . 10.1006/geno.2002.6776 .
3. Nowotny M, Gaidamakov SA, Ghirlando R, Cerritelli SM, Crouch RJ, Yang W . Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription . Mol. Cell . 28 . 2 . 264–76 . October 2007 . 17964265 . 10.1016/j.molcel.2007.08.015 . free .