RNASE1 explained

Ribonuclease pancreatic is an enzyme that in humans is encoded by the RNASE1 gene.^{[1] [2]}

Function

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A super-family. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyses 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ss-RNA over ds-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.^[2]

Further reading

• Nogués MV, Vilanova M, Cuchillo CM . Bovine pancreatic ribonuclease A as a model of an enzyme with multiple substrate binding sites . Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology . 1253 . 1 . 16–24 . Nov 1995 . 7492594 . 10.1016/0167-4838(95)00138-k .
• Schienman JE, Holt RA, Auerbach MR, Stewart CB . Duplication and divergence of 2 distinct pancreatic ribonuclease genes in leaf-eating African and Asian colobine monkeys . Molecular Biology and Evolution . 23 . 8 . 1465–79 . Aug 2006 . 16751256 . 10.1093/molbev/msl025 .
• Sakakibara R, Hashida K, Kitahara T, Ishiguro M . Characterization of a unique nonsecretory ribonuclease from urine of pregnant women . Journal of Biochemistry . 111 . 3 . 325–30 . Mar 1992 . 1587793 . 10.1093/oxfordjournals.jbchem.a123757.
• Haugg M, Schein CH . The DNA sequences of the human and hamster secretory ribonucleases determined with the polymerase chain reaction (PCR) . Nucleic Acids Research . 20 . 3 . 612 . Feb 1992 . 1741299 . 310435 . 10.1093/nar/20.3.612 .
• Sakakibara R, Hashida K, Tominaga N, Sakai K, Ishiguro M, Imamura S, Ohmatsu F, Sato E . A putative mouse oocyte maturation inhibitory protein from urine of pregnant women: N-terminal sequence homology with human nonsecretory ribonuclease . Chemical & Pharmaceutical Bulletin . 39 . 1 . 146–9 . Jan 1991 . 2049798 . 10.1248/cpb.39.146 . free .
• Mizuta K, Awazu S, Yasuda T, Kishi K . Purification and characterization of three ribonucleases from human kidney: comparison with urine ribonucleases . Archives of Biochemistry and Biophysics . 281 . 1 . 144–51 . Aug 1990 . 2383019 . 10.1016/0003-9861(90)90424-W .
• Beintema JJ, Blank A, Schieven GL, Dekker CA, Sorrentino S, Libonati M . Differences in glycosylation pattern of human secretory ribonucleases . The Biochemical Journal . 255 . 2 . 501–5 . Oct 1988 . 3202829 . 1135256 .
• Beintema JJ, Wietzes P, Weickmann JL, Glitz DG . The amino acid sequence of human pancreatic ribonuclease . Analytical Biochemistry . 136 . 1 . 48–64 . Jan 1984 . 6201087 . 10.1016/0003-2697(84)90306-3 .
• Russo N, de Nigris M, Ciardiello A, Di Donato A, D'Alessio G . Expression in mammalian cells, purification and characterization of recombinant human pancreatic ribonuclease . FEBS Letters . 369 . 2–3 . 352 . Aug 1995 . 7649283 . 10.1016/0014-5793(95)96890-G . 45739736 .
• Seno M, Futami J, Kosaka M, Seno S, Yamada H . Nucleotide sequence encoding human pancreatic ribonuclease . Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression . 1218 . 3 . 466–8 . Aug 1994 . 8049276 . 10.1016/0167-4781(94)90208-9 .
• Yasuda T, Nadano D, Takeshita H, Kishi K . Two distinct secretory ribonucleases from human cerebrum: purification, characterization and relationships to other ribonucleases . The Biochemical Journal . 296 . 3 . 617–25 . Dec 1993 . 8280059 . 1137742 . 10.1042/bj2960617.
• Papageorgiou AC, Shapiro R, Acharya KR . Molecular recognition of human angiogenin by placental ribonuclease inhibitor--an X-ray crystallographic study at 2.0 A resolution . The EMBO Journal . 16 . 17 . 5162–77 . Sep 1997 . 9311977 . 1170149 . 10.1093/emboj/16.17.5162 .
• Klink TA, Woycechowsky KJ, Taylor KM, Raines RT . Contribution of disulfide bonds to the conformational stability and catalytic activity of ribonuclease A . European Journal of Biochemistry . 267 . 2 . 566–72 . Jan 2000 . 10632727 . 10.1046/j.1432-1327.2000.01037.x . free .
• Pous J, Canals A, Terzyan SS, Guasch A, Benito A, Ribó M, Vilanova M, Coll M . Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases . Journal of Molecular Biology . 303 . 1 . 49–60 . Oct 2000 . 11021969 . 10.1006/jmbi.2000.4506 .
• Pous J, Mallorquí-Fernández G, Peracaula R, Terzyan SS, Futami J, Tada H, Yamada H, Seno M, de Llorens R, Gomis-Rüth FX, Coll M . Three-dimensional structure of human RNase 1 delta N7 at 1.9 A resolution . Acta Crystallographica Section D . 57 . Pt 4 . 498–505 . Apr 2001 . 11264578 . 10.1107/S0907444901001147 . 2001AcCrD..57..498P .
• Gaur D, Swaminathan S, Batra JK . Interaction of human pancreatic ribonuclease with human ribonuclease inhibitor. Generation of inhibitor-resistant cytotoxic variants . The Journal of Biological Chemistry . 276 . 27 . 24978–84 . Jul 2001 . 11342552 . 10.1074/jbc.M102440200 . free .
• Futami J, Maeda T, Kitazoe M, Nukui E, Tada H, Seno M, Kosaka M, Yamada H . Preparation of potent cytotoxic ribonucleases by cationization: enhanced cellular uptake and decreased interaction with ribonuclease inhibitor by chemical modification of carboxyl groups . Biochemistry . 40 . 25 . 7518–24 . Jun 2001 . 11412105 . 10.1021/bi010248g .
• Canals A, Pous J, Guasch A, Benito A, Ribó M, Vilanova M, Coll M . The structure of an engineered domain-swapped ribonuclease dimer and its implications for the evolution of proteins toward oligomerization . Structure . 9 . 10 . 967–76 . Oct 2001 . 11591351 . 10.1016/S0969-2126(01)00659-1 . free .

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human Ribonuclease pancreatic

Notes and References

1. Kochetov AV, Lukasheva VV, Filipenko ML, Mertvetsov NP, Rivkin MI . [Primary structure of the coding part of the gene for human pancreatic ribonuclease and its chromosomal location] . Bioorganicheskaia Khimiia . 21 . 9 . 691–4 . Sep 1995 . 8588814 .
2. Web site: Entrez Gene: RNASE1 ribonuclease, RNase A family, 1 (pancreatic).