RNA-3'-phosphate cyclase explained
| RNA-3′-phosphate cyclase |
| Ec Number: | 6.5.1.4 |
| Cas Number: | 85638-41-1 |
| Go Code: | 0003963 |
In enzymology, a RNA-3′-phosphate cyclase is an enzyme that catalyzes the chemical reaction
ATP + RNA 3'-terminal-phosphate
AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
Thus, the two substrates of this enzyme are ATP and RNA 3'-terminal-phosphate, whereas its 3 products are AMP, diphosphate, and RNA terminal-2',3'-cyclic-phosphate.
This enzyme belongs to the family of ligases, specifically those forming phosphoric-ester bonds. The systematic name of this enzyme class is RNA-3'-phosphate:RNA ligase (cyclizing, AMP-forming). This enzyme is also called RNA cyclase.
Structural studies
As of 2010, three structures have been solved for this class of enzymes, with PDB accession codes and, (un-adenylated)[1] and (adenylated).[2]
Further reading
- Filipowicz W, Konarska M, Gross HJ, Shatkin AJ . 1983 . RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa cell extract . Nucleic Acids Res. . 11 . 1405 - 18 . 6828385 . 10.1093/nar/11.5.1405 . 5 . 325805 .
- Reinberg D, Arenas J, Hurwitz J . 1985 . The enzymatic conversion of 3'-phosphate terminated RNA chains to 2',3'-cyclic phosphate derivatives . J. Biol. Chem. . 260 . 6088 - 97 . 2581947 . 10 . 10.1016/S0021-9258(18)88941-4 . free .
[3] [4]
Notes and References
- Palm GJ, Billy E, Filipowicz W, Wlodawer A . Crystal structure of RNA 3'-terminal phosphate cyclase, a ubiquitous enzyme with unusual topology . Structure . 8 . 1 . 13–23 . January 2000 . 10673421 . 10.1016/S0969-2126(00)00076-9. free .
- Tanaka N, Smith P, Shuman S . Structure of the RNA 3'-phosphate cyclase–adenylate intermediate illuminates nucleotide specificity and covalent nucleotidyl transfer . Structure . 18 . 4 . 449–57 . March 2010 . 20399182 . 2858066 . 10.1016/j.str.2010.01.016 .
- RNA 3'-terminal phosphate cyclases and cyclase-like proteins. 28132487. 2016. Filipowicz. W.. Postepy Biochemii. 62. 3. 327–334. 10.18388/pb.2016_32 . 36153563 . free.
- 10.1016/S0969-2126(00)00076-9. Crystal structure of RNA 3′-terminal phosphate cyclase, a ubiquitous enzyme with unusual topology. 2000. Palm. Gottfried J.. Billy. Eric. Filipowicz. Witold. Wlodawer. Alexander. Structure. 8. 1. 13–23. 10673421. free.
