Rhamnogalacturonan endolyase explained

Rhamnogalacturonan endolyase
Ec Number:	4.2.2.23

The enzyme Rhamnogalacturonan endolyase (rhamnogalacturonase B, α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronide lyase, Rgase B, rhamnogalacturonan α-Lrhamnopyranosyl-(1,4)-α-D-galactopyranosyluronide lyase, RG-lyase, YesW, RGL4, Rgl11A, Rgl11Y, RhiE) is an enzyme with systematic name α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronate endolyase.^{[1] [2] [3] [4] [5] [6] [7] [8]} catalyses the following process:

Endotype eliminative cleavage of L-α-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated Dgalactopyranosyluronic acid at the non-reducing end.

The enzyme is part of the degradation system for rhamnogalacturonan I in Bacillus subtilis strain 168 and Aspergillus aculeatus.

Notes and References

1. Mutter M, Colquhoun IJ, Schols HA, Beldman G, Voragen AG . Rhamnogalacturonase B from Aspergillus aculeatus is a rhamnogalacturonan alpha-L-rhamnopyranosyl-(1-->4)-alpha-D-galactopyranosyluronide lyase . Plant Physiology . 110 . 1 . 73–7 . January 1996 . 8587995 . 157695 . 10.1104/pp.110.1.73 .
2. Azadi P, O'Neill MA, Bergmann C, Darvill AG, Albersheim P . The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase . Glycobiology . 5 . 8 . 783–9 . December 1995 . 8720076 . 10.1093/glycob/5.8.783 .
3. Mutter M, Colquhoun IJ, Beldman G, Schols HA, Bakx EJ, Voragen AG . Characterization of recombinant rhamnogalacturonan alpha-L-rhamnopyranosyl-(1,4)-alpha-D-galactopyranosyluronide lyase from Aspergillus aculeatus. An enzyme that fragments rhamnogalacturonan I regions of pectin . Plant Physiology . 117 . 1 . 141–52 . May 1998 . 9576783 . 34997 . 10.1104/pp.117.1.141 .
4. Kadirvelraj R, Harris P, Poulsen JC, Kauppinen S, Larsen S . A stepwise optimization of crystals of rhamnogalacturonan lyase from Aspergillus aculeatus . Acta Crystallographica Section D . 58 . Pt 8 . 1346–9 . August 2002 . 12136151 . 10.1107/s0907444902009137 .
5. Laatu M, Condemine G . Rhamnogalacturonate lyase RhiE is secreted by the out system in Erwinia chrysanthemi . Journal of Bacteriology . 185 . 5 . 1642–9 . March 2003 . 12591882 . 148073 . 10.1128/jb.185.5.1642-1649.2003 .
6. Pagès S, Valette O, Abdou L, Bélaïch A, Bélaïch JP . A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome . Journal of Bacteriology . 185 . 16 . 4727–33 . August 2003 . 12896991 . 166469 . 10.1128/jb.185.16.4727-4733.2003 .
7. Ochiai A, Yamasaki M, Itoh T, Mikami B, Hashimoto W, Murata K . Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11 . Acta Crystallographica Section F . 62 . Pt 5 . 438–40 . May 2006 . 16682770 . 2219969 . 10.1107/s1744309106011894 .
8. Jensen MH, Otten H, Christensen U, Borchert TV, Christensen LL, Larsen S, Leggio LL . Structural and biochemical studies elucidate the mechanism of rhamnogalacturonan lyase from Aspergillus aculeatus . Journal of Molecular Biology . 404 . 1 . 100–11 . November 2010 . 20851126 . 10.1016/j.jmb.2010.09.013 .