RBPJ explained

Recombination signal binding protein for immunoglobulin kappa J region is a protein that in humans is encoded by the RBPJ gene.^{[1] [2] [3]}

RBPJ^[4] also known as CBF1, is the human homolog for the Drosophila gene Suppressor of Hairless. Its promoter region is classically used to demonstrate Notch1 signaling.^[5]

Interactions

RBPJ has been shown to interact with:

• NOTCH1^{[6] [7]}
• NCOR2,^{[8] [9]}
• PCAF,^[10]
• SND1,^[11]
• SNW1,^{[12] [13]}
• SOX18,^[14] and
• HIF1a. ^[15]

Further reading

• Oka C, Kawaichi M . V(D)J recombination of immunoglobulin genes . Advances in Biophysics . 31 . 163–180 . 1995 . 7625272 . 10.1016/0065-227X(95)99390-B .
• Matsunami N, Hamaguchi Y, Yamamoto Y, Kuze K, Kangawa K, Matsuo H, Kawaichi M, Honjo T . A protein binding to the J kappa recombination sequence of immunoglobulin genes contains a sequence related to the integrase motif . Nature . 342 . 6252 . 934–937 . 1990 . 2556644 . 10.1038/342934a0 . 4370462 .
• Tong X, Drapkin R, Yalamanchili R, Mosialos G, Kieff E . The Epstein-Barr virus nuclear protein 2 acidic domain forms a complex with a novel cellular coactivator that can interact with TFIIE . Molecular and Cellular Biology . 15 . 9 . 4735–4744 . September 1995 . 7651391 . 230717 . 10.1128/mcb.15.9.4735 .
• Zhao B, Marshall DR, Sample CE . A conserved domain of the Epstein-Barr virus nuclear antigens 3A and 3C binds to a discrete domain of Jkappa . Journal of Virology . 70 . 7 . 4228–4236 . July 1996 . 8676443 . 190353 . 10.1128/JVI.70.7.4228-4236.1996 .
• Tamura K, Taniguchi Y, Minoguchi S, Sakai T, Tun T, Furukawa T, Honjo T . Physical interaction between a novel domain of the receptor Notch and the transcription factor RBP-J kappa/Su(H) . Current Biology . 5 . 12 . 1416–1423 . December 1995 . 8749394 . 10.1016/S0960-9822(95)00279-X . 1995CBio....5.1416T . free . 18442572 . 2433/202204 .
• Gress TM, Müller-Pillasch F, Geng M, Zimmerhackl F, Zehetner G, Friess H, Büchler M, Adler G, Lehrach H . A pancreatic cancer-specific expression profile . Oncogene . 13 . 8 . 1819–1830 . October 1996 . 8895530 .
• Hsieh JJ, Nofziger DE, Weinmaster G, Hayward SD . Epstein-Barr virus immortalization: Notch2 interacts with CBF1 and blocks differentiation . Journal of Virology . 71 . 3 . 1938–1945 . March 1997 . 9032325 . 191274 . 10.1128/JVI.71.3.1938-1945.1997 .
• Aster JC, Robertson ES, Hasserjian RP, Turner JR, Kieff E, Sklar J . Oncogenic forms of NOTCH1 lacking either the primary binding site for RBP-Jkappa or nuclear localization sequences retain the ability to associate with RBP-Jkappa and activate transcription . The Journal of Biological Chemistry . 272 . 17 . 11336–11343 . April 1997 . 9111040 . 10.1074/jbc.272.17.11336 . free .
• Kao HY, Ordentlich P, Koyano-Nakagawa N, Tang Z, Downes M, Kintner CR, Evans RM, Kadesch T . A histone deacetylase corepressor complex regulates the Notch signal transduction pathway . Genes & Development . 12 . 15 . 2269–2277 . August 1998 . 9694793 . 317043 . 10.1101/gad.12.15.2269 .
• Hsieh JJ, Zhou S, Chen L, Young DB, Hayward SD . CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex . Proceedings of the National Academy of Sciences of the United States of America . 96 . 1 . 23–28 . January 1999 . 9874765 . 15086 . 10.1073/pnas.96.1.23 . free . 1999PNAS...96...23H .
• Scanlan MJ, Gordan JD, Williamson B, Stockert E, Bander NH, Jongeneel V, Gure AO, Jäger D, Jäger E, Knuth A, Chen YT, Old LJ . Antigens recognized by autologous antibody in patients with renal-cell carcinoma . International Journal of Cancer . 83 . 4 . 456–464 . November 1999 . 10508479 . 10.1002/(SICI)1097-0215(19991112)83:4<456::AID-IJC4>3.0.CO;2-5 . free .
• Callahan J, Aster J, Sklar J, Kieff E, Robertson ES . Intracellular forms of human NOTCH1 interact at distinctly different levels with RBP-jkappa in human B and T cells . Leukemia . 14 . 1 . 84–92 . January 2000 . 10637481 . 10.1038/sj.leu.2401630 . free .
• Smith PR, de Jesus O, Turner D, Hollyoake M, Karstegl CE, Griffin BE, Karran L, Wang Y, Hayward SD, Farrell PJ . Structure and coding content of CST (BART) family RNAs of Epstein-Barr virus . Journal of Virology . 74 . 7 . 3082–3092 . April 2000 . 10708423 . 111807 . 10.1128/JVI.74.7.3082-3092.2000 .
• Zhou S, Fujimuro M, Hsieh JJ, Chen L, Miyamoto A, Weinmaster G, Hayward SD . SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function . Molecular and Cellular Biology . 20 . 7 . 2400–2410 . April 2000 . 10713164 . 85419 . 10.1128/MCB.20.7.2400-2410.2000 .
• Kurooka H, Honjo T . Functional interaction between the mouse notch1 intracellular region and histone acetyltransferases PCAF and GCN5 . The Journal of Biological Chemistry . 275 . 22 . 17211–17220 . June 2000 . 10747963 . 10.1074/jbc.M000909200 . free .
• Beatus P, Lundkvist J, Oberg C, Pedersen K, Lendahl U . The origin of the ankyrin repeat region in Notch intracellular domains is critical for regulation of HES promoter activity . Mechanisms of Development . 104 . 1–2 . 3–20 . June 2001 . 11404076 . 10.1016/S0925-4773(01)00373-2 . 9526831 . free .
• Zhou S, Hayward SD . Nuclear localization of CBF1 is regulated by interactions with the SMRT corepressor complex . Molecular and Cellular Biology . 21 . 18 . 6222–6232 . September 2001 . 11509665 . 87339 . 10.1128/MCB.21.18.6222-6232.2001 .

Notes and References

1. Amakawa R, Jing W, Ozawa K, Matsunami N, Hamaguchi Y, Matsuda F, Kawaichi M, Honjo T . Human Jk recombination signal binding protein gene (IGKJRB): comparison with its mouse homologue . Genomics . 17 . 2 . 306–315 . August 1993 . 8406481 . 10.1006/geno.1993.1326 .
2. Tang X, Saito-Ohara F, Song J, Koga C, Ugai H, Murakami H, Ikeuchi T, Yokoyama KK . Assignment of the human gene for KBF2/RBP-Jk to chromosome 9p12-13 and 9q13 by fluorescence in situ hybridization . The Japanese Journal of Human Genetics . 42 . 2 . 337–341 . June 1997 . 9290259 . 10.1007/BF02766956 . free .
3. Web site: NCBI Gene: RBPJ recombination signal binding protein for immunoglobulin kappa J region.
4. Web site: HGNC: RBPJ Symbol Report .
5. Hsieh JJ, Henkel T, Salmon P, Robey E, Peterson MG, Hayward SD . Truncated mammalian Notch1 activates CBF1/RBPJk-repressed genes by a mechanism resembling that of Epstein-Barr virus EBNA2 . Molecular and Cellular Biology . 16 . 3 . 952–959 . March 1996 . 8622698 . 231077 . 10.1128/mcb.16.3.952 .
6. Nam Y, Weng AP, Aster JC, Blacklow SC . Structural requirements for assembly of the CSL.intracellular Notch1.Mastermind-like 1 transcriptional activation complex . The Journal of Biological Chemistry . 278 . 23 . 21232–21239 . June 2003 . 12644465 . 10.1074/jbc.M301567200 . free .
7. Aster JC, Robertson ES, Hasserjian RP, Turner JR, Kieff E, Sklar J . Oncogenic forms of NOTCH1 lacking either the primary binding site for RBP-Jkappa or nuclear localization sequences retain the ability to associate with RBP-Jkappa and activate transcription . The Journal of Biological Chemistry . 272 . 17 . 11336–11343 . April 1997 . 9111040 . 10.1074/jbc.272.17.11336 . free .
8. Beatus P, Lundkvist J, Oberg C, Pedersen K, Lendahl U . The origin of the ankyrin repeat region in Notch intracellular domains is critical for regulation of HES promoter activity . Mechanisms of Development . 104 . 1–2 . 3–20 . June 2001 . 11404076 . 10.1016/s0925-4773(01)00373-2 . 9526831 . free .
9. Zhou S, Hayward SD . Nuclear localization of CBF1 is regulated by interactions with the SMRT corepressor complex . Molecular and Cellular Biology . 21 . 18 . 6222–6232 . September 2001 . 11509665 . 87339 . 10.1128/mcb.21.18.6222-6232.2001 .
10. Kurooka H, Honjo T . Functional interaction between the mouse notch1 intracellular region and histone acetyltransferases PCAF and GCN5 . The Journal of Biological Chemistry . 275 . 22 . 17211–17220 . June 2000 . 10747963 . 10.1074/jbc.M000909200 . free .
11. Hsieh JJ, Zhou S, Chen L, Young DB, Hayward SD . CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex . Proceedings of the National Academy of Sciences of the United States of America . 96 . 1 . 23–28 . January 1999 . 9874765 . 15086 . 10.1073/pnas.96.1.23 . free . 1999PNAS...96...23H .
12. Zhou S, Fujimuro M, Hsieh JJ, Chen L, Hayward SD . A role for SKIP in EBNA2 activation of CBF1-repressed promoters . Journal of Virology . 74 . 4 . 1939–1947 . February 2000 . 10644367 . 111672 . 10.1128/jvi.74.4.1939-1947.2000 .
13. Zhou S, Fujimuro M, Hsieh JJ, Chen L, Miyamoto A, Weinmaster G, Hayward SD . SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function . Molecular and Cellular Biology . 20 . 7 . 2400–2410 . April 2000 . 10713164 . 85419 . 10.1128/mcb.20.7.2400-2410.2000 .
14. Overman J, Fontaine F, Wylie-Sears J, Moustaqil M, Huang L, Meurer M, Chiang IK, Lesieur E, Patel J, Zuegg J, Pasquier E, Sierecki E, Gambin Y, Hamdan M, Khosrotehrani K, Andelfinger G, Bischoff J, Francois M . R-propranolol is a small molecule inhibitor of the SOX18 transcription factor in a rare vascular syndrome and hemangioma . eLife . 8 . e43026 . July 2019 . 31358114 . 6667216 . 10.7554/eLife.43026 . van Lohuizen M, Koh GY, Ostergaard P . free .
15. Díaz-Trelles R, Scimia MC, Bushway P, Tran D, Monosov A, Monosov E, Peterson K, Rentschler S, Cabrales P, Ruiz-Lozano P, Mercola M . Notch-independent RBPJ controls angiogenesis in the adult heart . Nature Communications . 7 . 1 . 12088 . June 2016 . 27357444 . 4931341 . 10.1038/ncomms12088 . 2016NatCo...712088D .