RASGRP3 explained
Ras guanyl-releasing protein 3 is a protein that in humans is encoded by the RASGRP3 gene.[1] [2] [3]
Function
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000).[3]
Interactions
RASGRP3 has been shown to interact with PRKCD.[4]
Further reading
- Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination . Genome Research . 10 . 11 . 1788–95 . Nov 2000 . 11076863 . 310948 . 10.1101/gr.143000 .
- Lorenzo PS, Kung JW, Bottorff DA, Garfield SH, Stone JC, Blumberg PM . Phorbol esters modulate the Ras exchange factor RasGRP3 . Cancer Research . 61 . 3 . 943–9 . Feb 2001 . 11221888 .
- Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Research . 11 . 3 . 422–35 . Mar 2001 . 11230166 . 311072 . 10.1101/gr.GR1547R .
- Teixeira C, Stang SL, Zheng Y, Beswick NS, Stone JC . Integration of DAG signaling systems mediated by PKC-dependent phosphorylation of RasGRP3 . Blood . 102 . 4 . 1414–20 . Aug 2003 . 12730099 . 10.1182/blood-2002-11-3621 . free .
- Brodie C, Steinhart R, Kazimirsky G, Rubinfeld H, Hyman T, Ayres JN, Hur GM, Toth A, Yang D, Garfield SH, Stone JC, Blumberg PM . PKCdelta associates with and is involved in the phosphorylation of RasGRP3 in response to phorbol esters . Molecular Pharmacology . 66 . 1 . 76–84 . Jul 2004 . 15213298 . 10.1124/mol.66.1.76 . 5774849 .
- Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A . From ORFeome to biology: a functional genomics pipeline . Genome Research . 14 . 10B . 2136–44 . Oct 2004 . 15489336 . 528930 . 10.1101/gr.2576704 .
- Aiba Y, Oh-hora M, Kiyonaka S, Kimura Y, Hijikata A, Mori Y, Kurosaki T . Activation of RasGRP3 by phosphorylation of Thr-133 is required for B cell receptor-mediated Ras activation . Proceedings of the National Academy of Sciences of the United States of America . 101 . 47 . 16612–7 . Nov 2004 . 15545601 . 528733 . 10.1073/pnas.0407468101 . 2004PNAS..10116612A . free .
- Roberts DM, Anderson AL, Hidaka M, Swetenburg RL, Patterson C, Stanford WL, Bautch VL . A vascular gene trap screen defines RasGRP3 as an angiogenesis-regulated gene required for the endothelial response to phorbol esters . Molecular and Cellular Biology . 24 . 24 . 10515–28 . Dec 2004 . 15572660 . 533983 . 10.1128/MCB.24.24.10515-10528.2004 .
- Zheng Y, Liu H, Coughlin J, Zheng J, Li L, Stone JC . Phosphorylation of RasGRP3 on threonine 133 provides a mechanistic link between PKC and Ras signaling systems in B cells . Blood . 105 . 9 . 3648–54 . May 2005 . 15657177 . 10.1182/blood-2004-10-3916 . free .
- Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S . The LIFEdb database in 2006 . Nucleic Acids Research . 34 . Database issue . D415-8 . Jan 2006 . 16381901 . 1347501 . 10.1093/nar/gkj139 .
- Okamura SM, Oki-Idouchi CE, Lorenzo PS . The exchange factor and diacylglycerol receptor RasGRP3 interacts with dynein light chain 1 through its C-terminal domain . The Journal of Biological Chemistry . 281 . 47 . 36132–9 . Nov 2006 . 17012239 . 10.1074/jbc.M605093200 . free .
- Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . Large-scale mapping of human protein-protein interactions by mass spectrometry . Molecular Systems Biology . 3 . 1 . 89 . 2007 . 17353931 . 1847948 . 10.1038/msb4100134 .
- Randhawa PK, Rylova S, Heinz JY, Kiser S, Fried JH, Dunworth WP, Anderson AL, Barber AT, Chappell JC, Roberts DM, Bautch VL . The Ras activator RasGRP3 mediates diabetes-induced embryonic defects and affects endothelial cell migration . Circulation Research . 108 . 10 . 1199–208 . May 2011 . 21474816 . 10.1161/CIRCRESAHA.110.230888 . 3709466 .
Notes and References
- Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O . Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro . DNA Research . 5 . 6 . 355–64 . Dec 1998 . 10048485 . 10.1093/dnares/5.6.355 . free .
- Rebhun JF, Castro AF, Quilliam LA . Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction . The Journal of Biological Chemistry . 275 . 45 . 34901–8 . Nov 2000 . 10934204 . 10.1074/jbc.M005327200 . free .
- Web site: Entrez Gene: RASGRP3 RAS guanyl releasing protein 3 (calcium and DAG-regulated).
- Brodie C, Steinhart R, Kazimirsky G, Rubinfeld H, Hyman T, Ayres JN, Hur GM, Toth A, Yang D, Garfield SH, Stone JC, Blumberg PM . PKCdelta associates with and is involved in the phosphorylation of RasGRP3 in response to phorbol esters . Molecular Pharmacology . 66 . 1 . 76–84 . Jul 2004 . 15213298 . 10.1124/mol.66.1.76 . 5774849 .