RANBP1 explained

Ran-specific binding protein 1 is an enzyme that in humans is encoded by the RANBP1 gene.^{[1] [2] [3]}

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RANGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Interactions

RANBP1 has been shown to interact with XPO1,^{[4] [5]} KPNB1^{[5] [6]} and Ran.^{[5] [7] [8]}

Further reading

• Bischoff FR . Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1 . EMBO J. . 14 . 4 . 705–15 . 1995 . 7882974 . 10.1002/j.1460-2075.1995.tb07049.x. 398135 . vanc. Krebber H . Smirnova E . 3 . Dong . W . Ponstingl . H .
• Ren M . Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing . Mol. Cell. Biol. . 15 . 4 . 2117–24 . 1995 . 7891706 . 10.1128/MCB.15.4.2117. 230439 . vanc. Villamarin A . Shih A . 3 . Coutavas . E . Moore . MS . Locurcio . M . Clarke . V . Oppenheim . JD . d'Eustachio . P .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Coutavas E . Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4 . Nature . 366 . 6455 . 585–7 . 1994 . 8255297 . 10.1038/366585a0 . vanc. Ren M . Oppenheim JD . 3 . d'Eustachio . Peter . Rush . Mark G. . 1993Natur.366..585C. 4302198 .
• Zolotukhin AS, Felber BK . Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1 . J. Biol. Chem. . 272 . 17 . 11356–60 . 1997 . 9111043 . 10.1074/jbc.272.17.11356 . free .
• Suzuki Y . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 . vanc. Yoshitomo-Nakagawa K . Maruyama K . 3 . Suyama . Akira . Sugano . Sumio .
• Plafker K, Macara IG . Facilitated Nucleocytoplasmic Shuttling of the Ran Binding Protein RanBP1 . Mol. Cell. Biol. . 20 . 10 . 3510–21 . 2000 . 10779340 . 10.1128/MCB.20.10.3510-3521.2000 . 85643 .
• Steggerda SM, Paschal BM . The mammalian Mog1 protein is a guanine nucleotide release factor for Ran . J. Biol. Chem. . 275 . 30 . 23175–80 . 2000 . 10811801 . 10.1074/jbc.C000252200 . free .
• Maynard TM . RanBP1, a velocardiofacial/DiGeorge syndrome candidate gene, is expressed at sites of mesenchymal/epithelial induction . Mech. Dev. . 111 . 1–2 . 177–80 . 2002 . 11804793 . 10.1016/S0925-4773(01)00616-5 . vanc. Haskell GT . Bhasin N . 3 . Lee . James M . Gassman . Andrew A . Lieberman . Jeffrey A . Lamantia . Anthony-Samuel . free .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . vanc. Feingold EA . Grouse LH . 3 . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M. free .
• Di Fiore B . Mammalian RanBP1 regulates centrosome cohesion during mitosis . J. Cell Sci. . 116 . Pt 16 . 3399–411 . 2004 . 12840069 . 10.1242/jcs.00624 . vanc. Ciciarello M . Mangiacasale R . 3 . Palena . A . Tassin . AM . Cundari . E . Lavia . P . free .
• Seewald MJ . Biochemical Characterization of the Ran-RanBP1-RanGAP System: Are RanBP Proteins and the Acidic Tail of RanGAP Required for the Ran-RanGAP GTPase Reaction? . Mol. Cell. Biol. . 23 . 22 . 8124–36 . 2003 . 14585972 . 10.1128/MCB.23.22.8124-8136.2003 . 262373 . vanc. Kraemer A . Farkasovsky M . 3 . Korner . C. . Wittinghofer . A. . Vetter . I. R. .
• Ota T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . vanc. Suzuki Y . Nishikawa T . 3 . Otsuki . Tetsuji . Sugiyama . Tomoyasu . Irie . Ryotaro . Wakamatsu . Ai . Hayashi . Koji . Sato . Hiroyuki . free .
• Bohlson SS, Zhang M, Ortiz CE, Tenner AJ . CD93 interacts with the PDZ domain-containing adaptor protein GIPC: implications in the modulation of phagocytosis . J. Leukoc. Biol. . 77 . 1 . 80–9 . 2005 . 15459234 . 10.1189/jlb.0504305 . 3219168 .
• Collins JE . A genome annotation-driven approach to cloning the human ORFeome . Genome Biol. . 5 . 10 . R84 . 2005 . 15461802 . 10.1186/gb-2004-5-10-r84 . 545604 . vanc. Wright CL . Edwards CA . 3 . Davis . Matthew P . Grinham . James A . Cole . Charlotte G . Goward . Melanie E . Aguado . Begoña . Mallya . Meera . free .
• Bruneel A . Proteomics of human umbilical vein endothelial cells applied to etoposide-induced apoptosis . Proteomics . 5 . 15 . 3876–84 . 2006 . 16130169 . 10.1002/pmic.200401239 . vanc. Labas V . Mailloux A . 3 . Sharma . Sanjiv . Royer . Nicolas . Vinh . Joëlle . Pernet . Pascal . Vaubourdolle . Michel . Baudin . Bruno . 26007149 .
• Takeda E, Hieda M, Katahira J, Yoneda Y . Phosphorylation of RanGAP1 stabilizes its interaction with Ran and RanBP1 . Cell Struct. Funct. . 30 . 2 . 69–80 . 2006 . 16428860 . 10.1247/csf.30.69 . free .
• Ewing RM . Large-scale mapping of human protein–protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 . vanc. Chu P . Elisma F . 3 . Li . Hongyan . Taylor . Paul . Climie . Shane . McBroom-Cerajewski . Linda . Robinson . Mark D . O'Connor . Liam .

Notes and References

1. Hayashi N, Yokoyama N, Seki T, Azuma Y, Ohba T, Nishimoto T . RanBP1, a Ras-like nuclear G protein binding to Ran/TC4, inhibits RCC1 via Ran/TC4 . Mol Gen Genet . 247 . 6 . 661–9 . August 1995 . 7616957 . 10.1007/BF00290397 . 24266692 .
2. Kehlenbach RH, Dickmanns A, Kehlenbach A, Guan T, Gerace L . A Role for RanBP1 in the Release of CRM1 from the Nuclear Pore Complex in a Terminal Step of Nuclear Export . J Cell Biol . 145 . 4 . 645–57 . July 1999 . 10330396 . 2133185 . 10.1083/jcb.145.4.645 .
3. Web site: Entrez Gene: RANBP1 RAN binding protein 1.
4. Singh . B B . Patel H H . Roepman R . Schick D . Ferreira P A . December 1999 . The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1 . J. Biol. Chem. . 274 . 52 . 37370–8 . 0021-9258. 10601307 . 10.1074/jbc.274.52.37370 . free . 2066/120791 . free .
5. Plafker . K . Macara I G . May 2000 . Facilitated Nucleocytoplasmic Shuttling of the Ran Binding Protein RanBP1 . Mol. Cell. Biol. . 20 . 10 . 3510–21 . 0270-7306. 10779340 . 10.1128/MCB.20.10.3510-3521.2000 . 85643 .
6. Yaseen . N R . Blobel G . September 1999 . GTP hydrolysis links initiation and termination of nuclear import on the nucleoporin nup358 . J. Biol. Chem. . 274 . 37 . 26493–502 . 0021-9258. 10473610 . 10.1074/jbc.274.37.26493 . free .
7. Ren . M . Villamarin A . Shih A . Coutavas E . Moore M S . LoCurcio M . Clarke V . Oppenheim J D . D'Eustachio P . Rush M G . April 1995 . Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing . Mol. Cell. Biol. . 15 . 4 . 2117–24 . 0270-7306. 7891706 . 230439 . 10.1128/MCB.15.4.2117 .
8. Steggerda . S M . Paschal B M . July 2000 . The mammalian Mog1 protein is a guanine nucleotide release factor for Ran . J. Biol. Chem. . 275 . 30 . 23175–80 . 0021-9258. 10811801 . 10.1074/jbc.C000252200 . free .