RAE1 explained

mRNA export factor is a protein that in humans is encoded by the RAE1 gene.^{[1] [2] [3]}

Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Interactions

RAE1 has been shown to interact with NUP98.^[4]

References

1. Bharathi A, Ghosh A, Whalen WA, Yoon JH, Pu R, Dasso M, Dhar R . The human RAE1 gene is a functional homologue of Schizosaccharomyces pombe rae1 gene involved in nuclear export of Poly(A)+ RNA . Gene . 198 . 1–2 . 251–8 . Dec 1997 . 9370289 . 10.1016/S0378-1119(97)00322-3 .
2. Kraemer D, Blobel G . mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm . Proc Natl Acad Sci U S A . 94 . 17 . 9119–24 . Sep 1997 . 9256445 . 23064 . 10.1073/pnas.94.17.9119 . 1997PNAS...94.9119K . free .
3. Web site: Entrez Gene: RAE1 RAE1 RNA export 1 homolog (S. pombe).
4. 10.1083/jcb.145.2.237 . Pritchard . C E . Fornerod M . Kasper L H . van Deursen J M . Apr 1999 . RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains . J. Cell Biol. . 145 . 2 . 237–54 . 0021-9525. 10209021 . 2133102 .

Further reading

• Nomura M, Zou Z, Joh T, etal . Genomic structures and characterization of Rae1 family members encoding GPI-anchored cell surface proteins and expressed predominantly in embryonic mouse brain. . J. Biochem. . 120 . 5 . 987–95 . 1997 . 8982867 . 10.1093/oxfordjournals.jbchem.a021517.
• Yoon JH, Whalen WA, Bharathi A, etal . Npp106p, a Schizosaccharomyces pombe nucleoporin similar to Saccharomyces cerevisiae Nic96p, functionally interacts with Rae1p in mRNA export. . Mol. Cell. Biol. . 17 . 12 . 7047–60 . 1997 . 9372936 . 10.1128/MCB.17.12.7047. 232561 .
• Matsuoka Y, Takagi M, Ban T, etal . Identification and characterization of nuclear pore subcomplexes in mitotic extract of human somatic cells. . Biochem. Biophys. Res. Commun. . 254 . 2 . 417–23 . 1999 . 9918853 . 10.1006/bbrc.1998.9953 .
• Pritchard CE, Fornerod M, Kasper LH, van Deursen JM . RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains. . J. Cell Biol. . 145 . 2 . 237–54 . 1999 . 10209021 . 10.1083/jcb.145.2.237 . 2133102 .
• Bachi A, Braun IC, Rodrigues JP, etal . The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates. . RNA . 6 . 1 . 136–58 . 2000 . 10668806 . 10.1017/S1355838200991994 . 1369901 .
• Yoon JH, Love DC, Guhathakurta A, etal . Mex67p of Schizosaccharomyces pombe interacts with Rae1p in mediating mRNA export. . Mol. Cell. Biol. . 20 . 23 . 8767–82 . 2000 . 11073978 . 10.1128/MCB.20.23.8767-8782.2000 . 86506 .
• Sabri N, Visa N . The Ct-RAE1 protein interacts with Balbiani ring RNP particles at the nuclear pore. . RNA . 6 . 11 . 1597–609 . 2000 . 11105759 . 10.1017/S1355838200001138 . 1370029 .
• Wang X, Babu JR, Harden JM, etal . The mitotic checkpoint protein hBUB3 and the mRNA export factor hRAE1 interact with GLE2p-binding sequence (GLEBS)-containing proteins. . J. Biol. Chem. . 276 . 28 . 26559–67 . 2001 . 11352911 . 10.1074/jbc.M101083200 . free .
• O'Callaghan CA, Cerwenka A, Willcox BE, etal . Molecular competition for NKG2D: H60 and RAE1 compete unequally for NKG2D with dominance of H60. . Immunity . 15 . 2 . 201–11 . 2001 . 11520456 . 10.1016/S1074-7613(01)00187-X . free .
• Deloukas P, Matthews LH, Ashurst J, etal . The DNA sequence and comparative analysis of human chromosome 20. . Nature . 414 . 6866 . 865–71 . 2002 . 11780052 . 10.1038/414865a . 2001Natur.414..865D . free .
• Kraemer D, Dresbach T, Drenckhahn D . Mrnp41 (Rae 1p) associates with microtubules in HeLa cells and in neurons. . Eur. J. Cell Biol. . 80 . 12 . 733–40 . 2002 . 11831386 . 10.1078/0171-9335-00216 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Faria PA, Chakraborty P, Levay A, etal . VSV disrupts the Rae1/mrnp41 mRNA nuclear export pathway. . Mol. Cell . 17 . 1 . 93–102 . 2005 . 15629720 . 10.1016/j.molcel.2004.11.023 . free .
• Benzinger A, Muster N, Koch HB, etal . Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer. . Mol. Cell. Proteomics . 4 . 6 . 785–95 . 2005 . 15778465 . 10.1074/mcp.M500021-MCP200 . free .
• Blower MD, Nachury M, Heald R, Weis K . A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. . Cell . 121 . 2 . 223–34 . 2005 . 15851029 . 10.1016/j.cell.2005.02.016 . 242561 . free .
• Wong RW, Blobel G, Coutavas E . Rae1 interaction with NuMA is required for bipolar spindle formation. . Proc. Natl. Acad. Sci. U.S.A. . 103 . 52 . 19783–7 . 2007 . 17172455 . 10.1073/pnas.0609582104 . 1750899 . 2006PNAS..10319783W . free .