| Country: | ZAF |
| Route: | 327 |
| Direction A: | South |
| Direction B: | North |
| Terminus A: | Mossel Bay |
| Terminus B: | Ladismith |
| Type: | R |
| Length Km: | 141 |
| Next Type: | R |
| Previous Type: | R |
| Previous Route: | 326 |
| Next Route: | 328 |
The R327 is a Regional Route in South Africa. Its a regional route in the Western Province and connects Mossel Bay to Ladismith and part of the northern route is a gravel road.
Its northern origin is from the R62 near Ladismith. From there it winds generally south-east. It goes over Cloete's Pass before reaching Herbertsdale. On the other side of the town it crosses the Du Plessis Pass. It then reaches its south-eastern terminus at the N2 near Mossel Bay.[1]