Quinolinate synthase explained

Quinolinate synthase
Ec Number:	2.5.1.72

Quinolinate synthase (NadA, QS, quinolinate synthetase) is an enzyme with systematic name glycerone phosphate:iminosuccinate alkyltransferase (cyclizing).^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

\rightleftharpoons pyridine-2,3-dicarboxylate + 2 H₂O + phosphate

This iron-sulfur protein that requires a [4Fe-4S] cluster for activity.

Notes and References

1. Ollagnier-de Choudens S, Loiseau L, Sanakis Y, Barras F, Fontecave M . Quinolinate synthetase, an iron-sulfur enzyme in NAD biosynthesis . FEBS Letters . 579 . 17 . 3737–43 . July 2005 . 15967443 . 10.1016/j.febslet.2005.05.065 . free .
2. Katoh A, Uenohara K, Akita M, Hashimoto T . Early steps in the biosynthesis of NAD in Arabidopsis start with aspartate and occur in the plastid . Plant Physiology . 141 . 3 . 851–7 . July 2006 . 16698895 . 1489895 . 10.1104/pp.106.081091 .
3. Sakuraba H, Tsuge H, Yoneda K, Katunuma N, Ohshima T . Crystal structure of the NAD biosynthetic enzyme quinolinate synthase . The Journal of Biological Chemistry . 280 . 29 . 26645–8 . July 2005 . 15937336 . 10.1074/jbc.C500192200 . free .
4. Rousset C, Fontecave M, Ollagnier de Choudens S . The [4Fe-4S] cluster of quinolinate synthase from Escherichia coli: investigation of cluster ligands . FEBS Letters . 582 . 19 . 2937–44 . August 2008 . 18674537 . 10.1016/j.febslet.2008.07.032 . free .
5. Saunders AH, Booker SJ . Regulation of the activity of Escherichia coli quinolinate synthase by reversible disulfide-bond formation . Biochemistry . 47 . 33 . 8467–9 . August 2008 . 18651751 . 3319134 . 10.1021/bi801135y .