| Province: | QC |
| Type: | QC |
| Route: | 216 |
| Maint: | Transports Québec |
| Map: | Route 216-QC.png |
| Length Km: | 315.5 |
| Length Round: | 1 |
| Length Ref: | [1] |
| Direction A: | West |
| Direction B: | East |
| Terminus A: | in Sainte-Catherine-de-Hatley |
| Terminus B: | in Sainte-Perpétue |
| Previous Type: | QC |
| Previous Route: | 215 |
| Next Type: | QC |
| Next Route: | 217 |
| Junction: | in Sherbrooke in Saint-Camille & Wotton in Saint-Adrien in Ham-Nord in Saint-Jacques-le-Majeur-de-Wolfestown in Irlande in Saint-Jean-de-Brébeuf in Kinnear's Mills in Saint-Jacques-de-Leeds in Sainte-Marie in Sainte-Marguerite in Saint-Malachie in Notre-Dame-Auxiliatrice-du-Buckland in Saint-Philémon in Saint-Paul-de-Montminy in Saint-Marcel |
Route 216 is a two-lane east/west highway in Quebec, Canada, which starts in Sainte-Catherine-de-Hatley in the Estrie region at the junction of Route 108 and ends in Sainte-Perpétue in Chaudière-Appalaches at the junction of Route 204.
Route 216 follows mostly a northeast/southwest course, and it is not a busy highway as it mostly links small villages between themselves in the backroads of the Appalachians. The only two major towns along the way are Sherbrooke and Sainte-Marie. Between Ham-Nord (junction with Route 161) and Saint-Jacques-le-Majeur-de-Wolfestown (junction with Route 263), the road is unpaved.