| Province: | QC |
| Type: | QC |
| Route: | 165 |
| Maint: | Transports Québec |
| Map: | Route 165-QC.png |
| Length Km: | 67.3 |
| Length Round: | 1 |
| Length Ref: | [1] |
| Direction A: | South |
| Direction B: | North |
| Terminus B: | in Saint-Louis-de-Blandford |
| Previous Type: | QC |
| Previous Route: | 162 |
| Next Type: | QC |
| Next Route: | 167 |
| Terminus A: | in Thetford Mines |
| Counties: | Les Appalaches / L'Érable / Arthabaska |
| Cities: | Thetford Mines |
| Junction: | in Irlande in Plessisville in Princeville in Saint-Louis-de-Blandford |
Route 165 is a 68 km north–south highway on the south shore of the Saint Lawrence River in Quebec, Canada. Its southern terminus is in Black Lake, now part of Thetford Mines, at the junction of Route 112 and its northern terminus is in Saint-Louis-de-Blandford at the junction of Autoroute 20. The stretch between Plessisville and Black Lake used to be Route 265 but it was re-numbered Route 165 in the 1990s.