| Province: | QC |
| Type: | Autoroute |
| Route: | 540 |
| Alternate Name: | Autoroute Duplessis |
| Maint: | Transports Québec |
| Map: | A-540.png |
| Length Km: | 5.1 |
| Length Round: | 1 |
| Length Ref: | [1] [2] |
| Direction A: | South |
| Terminus A: | in Québec |
| Junction: | in Québec |
| Direction B: | North |
| Terminus B: | in Québec |
| Cities: | Québec City |
| Established: | 1966 |
| Previous Type: | A |
| Previous Route: | 530 |
| Next Type: | A |
| Next Route: | 573 |
Autoroute 540 is a short spur highway, connecting Route 138 to Autoroute 73 and linking the Jean Lesage International Airport with the Pierre Laporte Bridge. It is also known as Autoroute Duplessis, honouring former Premier Maurice Duplessis.
Exit numbers on Autoroute 540 are numbered from north to south, unlike other Autoroutes which are numbered from south to north.