Putidaredoxin—NAD+ reductase explained

Putidaredoxin—NAD^+ reductase
Ec Number:	1.18.1.5

Putidaredoxin—NAD⁺ reductase (putidaredoxin reductase, camA (gene)) is an enzyme with systematic name putidaredoxin:NAD⁺ oxidoreductase.^{[1] [2] [3] [4] [5] [6] [7]} This enzyme catalyses the following chemical reaction

reduced putidaredoxin + NAD⁺

\rightleftharpoons oxidized putidaredoxin + NADH + H⁺

Putidaredoxin—NAD⁺ reductase requires FAD.

Notes and References

1. Roome PW, Philley JC, Peterson JA . Purification and properties of putidaredoxin reductase . The Journal of Biological Chemistry . 258 . 4 . 2593–8 . February 1983 . 6401738 .
2. Koga H, Yamaguchi E, Matsunaga K, Aramaki H, Horiuchi T . Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida . Journal of Biochemistry . 106 . 5 . 831–6 . November 1989 . 2613690 .
3. Peterson JA, Lorence MC, Amarneh B . Putidaredoxin reductase and putidaredoxin. Cloning, sequence determination, and heterologous expression of the proteins . The Journal of Biological Chemistry . 265 . 11 . 6066–73 . April 1990 . 2180940 .
4. Sevrioukova IF, Poulos TL . Putidaredoxin reductase, a new function for an old protein . The Journal of Biological Chemistry . 277 . 28 . 25831–9 . July 2002 . 12011076 . 10.1074/jbc.m201110200 . free .
5. Sevrioukova IF, Garcia C, Li H, Bhaskar B, Poulos TL . Crystal structure of putidaredoxin, the [2Fe-2S] component of the P450cam monooxygenase system from Pseudomonas putida . Journal of Molecular Biology . 333 . 2 . 377–92 . October 2003 . 14529624 . 10.1016/j.jmb.2003.08.028 .
6. Sevrioukova IF, Li H, Poulos TL . Crystal structure of putidaredoxin reductase from Pseudomonas putida, the final structural component of the cytochrome P450cam monooxygenase . Journal of Molecular Biology . 336 . 4 . 889–902 . February 2004 . 15095867 . 10.1016/j.jmb.2003.12.067 .
7. Smith N, Mayhew M, Holden MJ, Kelly H, Robinson H, Heroux A, Vilker VL, Gallagher DT . Structure of C73G putidaredoxin from Pseudomonas putida . Acta Crystallographica Section D . 60 . Pt 5 . 816–22 . May 2004 . 15103126 . 10.1107/s0907444904003348 .