TRNA pseudouridine55 synthase explained

tRNA pseudouridine^55 synthase
Ec Number:	5.4.99.25
Cas Number:	430429-15-5

tRNA pseudouridine⁵⁵ synthase (TruB, aCbf5, Pus4, YNL292w (gene), Psi55 tRNA pseudouridine synthase, tRNA:Psi⁵⁵-synthase, tRNA pseudouridine 55 synthase, tRNA:pseudouridine-55 synthase, Psi⁵⁵ synthase, tRNA Psi⁵⁵ synthase, tRNA:Psi⁵⁵ synthase, tRNA-uridine⁵⁵ uracil mutase, Pus10, tRNA-uridine^54/55 uracil mutase) is an enzyme with systematic name tRNA-uridine⁵⁵ uracil mutase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

tRNA uridine⁵⁵

\rightleftharpoons tRNA pseudouridine⁵⁵

Pseudouridine synthase TruB from Escherichia coli specifically modifies uridine⁵⁵ in tRNA molecules.

Notes and References

1. Nurse K, Wrzesinski J, Bakin A, Lane BG, Ofengand J . Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli . RNA . 1 . 1 . 102–12 . March 1995 . 7489483 . 1369054 .
2. Becker HF, Motorin Y, Planta RJ, Grosjean H . The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of psi55 in both mitochondrial and cytoplasmic tRNAs . Nucleic Acids Research . 25 . 22 . 4493–9 . November 1997 . 9358157 . 147073 . 10.1093/nar/25.22.4493 .
3. Pieńkowska J, Wrzesiński J, Szweykowska-Kulińska Z . A cell-free yellow lupin extract containing activities of pseudouridine 35 and 55 synthases . Acta Biochimica Polonica . 45 . 3 . 745–54 . 1998 . 10.18388/abp.1998_4268 . 9918501 . free .
4. Chaudhuri BN, Chan S, Perry LJ, Yeates TO . Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft . The Journal of Biological Chemistry . 279 . 23 . 24585–91 . June 2004 . 15028724 . 10.1074/jbc.m401045200 . free .
5. Hoang C, Hamilton CS, Mueller EG, Ferré-D'Amaré AR . Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain . Protein Science . 14 . 8 . 2201–6 . August 2005 . 15987897 . 2279332 . 10.1110/ps.051493605 .
6. Gurha P, Gupta R . Archaeal Pus10 proteins can produce both pseudouridine 54 and 55 in tRNA . RNA . 14 . 12 . 2521–7 . December 2008 . 18952823 . 2590954 . 10.1261/rna.1276508 .