PtK2 cells explained

PtK2 cells are a cell line derived from male long-nosed potoroo (Potorous tridactylis) epithelial kidney cells.[1] This cell line is used for a variety of applications in biomedical research but is particularly popular as a model for mitosis.[2]

Origin

The PtK2 cell line was established by Kirsten Walen and Spencer Brown in 1962.[3] Cells from the rat kangaroo were selected as the source of a cell line because this species has only a small number of chromosomes and these chromosomes are easily visualized under a microscope; mitosis becomes easy to observe, and the structural aspects of cell division can be closely examined.[4]

Characteristics

PtK2 cells are relatively large, and when grown in a monolayer, stay flat throughout the cell cycle - unlike many cells that round up during mitosis.[5] PtK2 cells are resistant to adenovirus 5, coxsackievirus B5, and poliovirus 2. They are susceptible to coxsackievirus A9, herpes simplex, vaccinia, and vesicular stomatitis (Ogden strain).[6] PtK2 cells contain intermediate filaments composed of Keratin.[7]

External links

Notes and References

  1. Web site: PtK2 (NBL-5) (ATCC® CCL-56™). 17 December 2013.
  2. Book: Elgin, edited by Barbara A. Hamkalo, Sarah C.R.. Functional organization of the nucleus a laboratory guide. 1991. Academic Press. San Diego. 008085933X. 143.
  3. Walen. KH. Brown, SW . Chromosomes in a marsupial (Potorous tridactylis) tissue culture.. Nature. Apr 28, 1962. 194. 4826 . 406. 14004541. 10.1038/194406a0. 1962Natur.194..406W . 4150008 . free.
  4. Web site: High Resolution Fluorescence Microscopy of PtK2 Cells Undergoing Mitosis in Microplates. BioTek. 24 June 2014. Peter J. Jr . Brescia . Peter . Banks. 20 June 2018.
  5. Web site: Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Fluorescence Digital Image Gallery - Male Rat Kangaroo Kidney Epithelial Cells (PtK2). 17 December 2013.
  6. Book: Steubing. Rosemarie. Lindl. Toni. Atlas of Living Cell Cultures. 25 March 2013 . Wiley. Weinheim. 978-3527669936. 465.
  7. Web site: Olympus Microscopy Resource Center Fluorescence Digital Image Gallery - Epithelial Cells. 17 December 2013.

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