Pseudotropine acyltransferase explained

Pseudotropine acyltransferase
Ec Number:	2.3.1.186
Cas Number:	138440-78-5, 162535-26-4

Pseudotropine acyltransferase (pseudotropine:acyl-CoA transferase, tigloyl-CoA:pseudotropine acyltransferase, acetyl-CoA:pseudotropine acyltransferase, pseudotropine acetyltransferase, pseudotropine tigloyltransferase, PAT) is an enzyme with systematic name acyl-CoA:pseudotropine O-acyltransferase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

\rightleftharpoons CoA + O-acylpseudotropine

This enzyme exhibits absolute specificity for the exo/3beta configuration found in pseudotropine as tropine (tropan-3alpha-ol).

Notes and References

1. Tigloyl-CoA:pseudotropine acyltransferase - an enzyme of tropane alkaloid biosynthesis . Rabot S, Peerless AC, Robins RJ . Phytochemistry . 1995 . 39 . 2 . 315–322 . 10.1016/0031-9422(94)00907-b.
2. Robins RJ, Bachmann P, Robinson T, Rhodes MJ, Yamada Y . The formation of 3 alpha- and 3 beta-acetoxytropanes by Datura stramonium transformed root cultures involves two acetyl-CoA-dependent acyltransferases . FEBS Letters . 292 . 1–2 . 293–7 . November 1991 . 1959620 . 10.1016/0014-5793(91)80887-9 . free .
3. Esterification reactions in the biosynthesis of tropane alkaloids in transformed root cultures . Robins RJ, Bachmann P, Peerless AC . Rabot, S. . Plant Cell Tissue Organ Cult. . 1994 . 38 . 2–3 . 241–247 . 10.1007/bf00033883. 21524758 .
4. Boswell HD, Dräger B, McLauchlan WR, Portsteffen A, Robins DJ, Robins RJ, Walton NJ . Specificities of the enzymes of N-alkyltropane biosynthesis in Brugmansia and Datura . Phytochemistry . 52 . 5 . 871–8 . November 1999 . 10626376 . 10.1016/S0031-9422(99)00293-9 .