Pseudomonas virus gh1 explained

Pseudomonas virus gh1 (Bacteriophage gh-1) is a bacteriophage capable of infecting susceptible strains of Pseudomonas putida.^[1] It is a member of family Podoviridae, subfamily Autographivirinae.^[2] It was first isolated in 1966 from a sample taken from the aeration tank at a sewage plant in East Lansing, Michigan.

Sedimentation analysis indicates that gh-1 carries its genetic payload in the form of a 37,359 bp linear strand of dsDNA,^[3] inside an icosahedronal capsid 50 nm in diameter.

One-step growth experiments indicate that the latent period is approximately 21 min, with a burst size of 103.

It has been shown that this phage group requires an intact O-antigen on its host's outer membrane in order to successfully replicate and it is thus likely that lipopolysaccharide acts as the phage receptor.^[4]

External links

• Web site: Pseudomonas putida bacteriophage gh-1 ATCC 12633-B1. - ATCC database entry for gh-1
• Web site: Pseudomonad phage gh-1. - Virus-Host Database

Notes and References

1. Lee . L. . Boezi . J. . Characterization of bacteriophage gh-1 for Pseudomonas putida. . Journal of Bacteriology . 92 . 6 . 1821–1827. 1966 . American Society for Microbiology . 10.1128/JB.92.6.1821-1827.1966 . 5958111 . 316266 . English.
2. Web site: Virus Taxonomy: 2018 Release . International Committee on Taxonomy of Viruses . 2018-12-07.
3. Lee . L. . Boezi . J. . Sedimentation Analysis of Pseudomonas putida A.3.12 Bacteriophage gh-1 Deoxyribonucleic Acid . Journal of Virology . 1 . 6 . 1274–1276. 1967 . American Society for Microbiology . 10.1128/JVI.1.6.1274-1276.1967 . English. 5621492 . 375419 .
4. Kovalyova . I. . Kropinski . A. . The complete genomic sequence of lytic bacteriophage gh-1 infecting Pseudomonas putida - Evidence for close relationship to the T7 group . Journal of Virology . 311 . 2 . 305–315. 2003 . 10.1016/S0042-6822(03)00124-7 . 12842620 . English. free .