Pseudomonas sRNA P16 explained
| Pseudomonas sRNA P16 |
| Symbol: | P16 |
| Rfam: | RF00628 |
| Rna Type: | Gene |
| Tax Domain: | Bacteria |
Pseudomonas sRNA P16 is a ncRNA that was predicted using bioinformatic tools in the genome of the opportunistic pathogen Pseudomonas aeruginosa and its expression verified by northern blot analysis.[1] P16 sRNA appears to be conserved across several Pseudomonas species and is consistently located downstream of a predicted TatD deoxyribonuclease gene. P16 has a predicted Rho independent terminator at the 3′ end but the function of P16 is unknown.
It has been shown that this sRNA is transcribed from an RpoS-dependent promoter under positive, probably indirect GacA control in two Pseudomonas species. It was renamed RgsA (for regulation by GacA and stress).[2] RpoS mRNA expression is repressed by RgsA during the exponential phase. The Hfq RNA chaperone is required for the repression.[3]
See also
Notes and References
- Livny J, Brencic A, Lory S, Waldor MK . Identification of 17 Pseudomonas aeruginosa sRNAs and prediction of sRNA-encoding genes in 10 diverse pathogens using the bioinformatic tool sRNAPredict2 . Nucleic Acids Res. . 34 . 12 . 3484–3493 . 2006 . 16870723 . 10.1093/nar/gkl453 . 1524904.
- González. Nicolas. Heeb. Stephan. Valverde. Claudio. Kay. Elisabeth. Reimmann. Cornelia. Junier. Thomas. Haas. Dieter. 2008-04-13. Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species. BMC Genomics. 9. 167. 10.1186/1471-2164-9-167. 1471-2164. 2375449. 18405392 . free .
- Lu. Pei. Wang. Yifei. Hu. Yangbo. Chen. Shiyun. April 2018. RgsA, an RpoS-dependent sRNA, negatively regulates rpoS expression in Pseudomonas aeruginosa. Microbiology. 164. 4. 716–724. 10.1099/mic.0.000632. 1465-2080. 29473822. free.
