Pseudomonas denitrificans explained

Pseudomonas denitrificans is a Gram-negative aerobic bacterium that performs denitrification. It was first isolated from garden soil in Vienna, Austria. It overproduces cobalamin (vitamin B₁₂), which it uses for methionine synthesis^[1] and it has been used for manufacture of the vitamin.^[2] Scientists at Rhône-Poulenc Rorer took a genetically engineered strain of the bacteria, in which eight of the cob genes involved in the biosynthesis of the vitamin had been overexpressed, to establish the complete sequence of methylation and other steps in the cobalamin pathway.^{[3] [4]}

Based on 16S rRNA analysis, P. denitrificans has been placed in the P. pertucinogena group.^[5]

External links

• Type strain of Pseudomonas denitrificans at BacDive - the Bacterial Diversity Metadatabase

Notes and References

1. Lago BD, and Demain AL . Alternate requirement for vitamin B₁₂ or methionine in mutants of Pseudomonas denitrificans, a vitamin B₁₂-producing bacterium . J Bacteriol . 99 . 1 . 347–9 . 1969 . 10.1128/jb.99.1.347-349.1969 . 5802615 . 250011 .
2. Fang . H . Kang . J . Zhang . D . Microbial production of vitamin B₁₂: a review and future perspectives. . Microbial Cell Factories . 30 January 2017 . 16 . 1 . 15 . 10.1186/s12934-017-0631-y . 28137297 . 5282855 . free .
3. 10.1128/jb.175.22.7430-7440.1993 . Biosynthesis of the corrin macrocycle of coenzyme B₁₂ in Pseudomonas denitrificans . 1993 . Debussche . L. . Thibaut . D. . Cameron . B. . Crouzet . J. . Blanche . F. . Journal of Bacteriology . 175 . 22 . 7430–7440 . 8226690 . 206888 .
4. Web site: Pathway: adenosylcobalamin biosynthesis II (aerobic) . R. Caspi . MetaCyc Metabolic Pathway Database . 2013-09-25 . 2020-04-24 .
5. 10.1099/00207713-50-4-1563 . Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence . 2000 . Anzai . Y. . Kim . H. . Park . J. Y. . Wakabayashi . H. . Oyaizu . H. . International Journal of Systematic and Evolutionary Microbiology . 50 . 4 . 1563–1589 . 10939664 .