| Prusly-sur-Ource | |
| Commune Status: | Commune |
| Image Coat Of Arms: | Blason de la ville de Prusly-sur-Ource (Côte-d'Or).svg |
| Arrondissement: | Montbard |
| Canton: | Châtillon-sur-Seine |
| Insee: | 21510 |
| Postal Code: | 21400 |
| Mayor: | Alain Verpy[1] |
| Term: | 2020 - 2026 |
| Intercommunality: | Pays Châtillonnais |
| Coordinates: | 47.8719°N 4.6622°W |
| Elevation M: | 240 |
| Elevation Min M: | 225 |
| Elevation Max M: | 320 |
| Area Km2: | 15.74 |
Prusly-sur-Ource (in French pronounced as /pʁyli syʁ uʁs/, literally Prusly on Ource) is a commune in the Côte-d'Or department in eastern France.