| Prienai District Municipality | |
| Native Name: | Prienų rajono savivaldybė |
| Native Name Lang: | lt |
| Settlement Type: | Municipality |
| Image Map1: | PrienuSen.png |
| Map Caption1: | Map of Prienai district municipality |
| Subdivision Type: | Country |
| Subdivision Name: |  Lithuania |
| Subdivision Type1: | Ethnographic region |
| Subdivision Name1: | Dzūkija / Suvalkija |
| Subdivision Type2: | County |
| Subdivision Name2: | Kaunas County |
| Parts Style: | para |
| Parts Type: | Elderships |
| P1: | 10 |
| Seat Type: | Capital |
| Seat: | Prienai |
| Unit Pref: | Metric |
| Area Total Km2: | 1031 |
| Area Rank: | 38th |
| Population Footnotes: | [1] |
| Population Total: | 25,286 |
| Population As Of: | 2021[2] |
| Population Rank: | 33rd |
| Population Density Km2: | auto |
| Population Density Rank: | 27th |
| Timezone1: | EET |
| Utc Offset1: | +2 |
| Timezone1 Dst: | EEST |
| Utc Offset1 Dst: | +3 |
| Area Code Type: | Telephone code |
| Area Code: | 319 |
| Blank Name Sec1: | Major settlements |
Prienai District Municipality is one of 60 municipalities in Lithuania. It was a member of the European Union-wide town twinning association Douzelage from 2008 to 2018.