| Preston N. Jacobus | |
| Office: | Mayor of Tucson, Arizona |
| Term Start: | 1910 |
| Term End: | 1911 |
| Predecessor: | Ben Heney |
| Successor: | Ira Erven Huffman |
| Birth Date: | 25 February 1864 |
| Birth Place: | Sussex County, New Jersey |
| Death Cause: | pulmonary tuberculosis |
| Residence: |
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| Spouse: | Anna C. Hedges |
| Party: | Republican Party |
Preston N. Jacobus (February 25, 1864 – November 28, 1911) was an American real estate developer, businessman, and politician. He served one term as Mayor of Tucson, Arizona Territory.[1] Jacobus was a member of the Republican Party.[2]