Premnaspirodiene oxygenase explained

Premnaspirodiene oxygenase
Ec Number:	1.14.13.121

Premnaspirodiene oxygenase (HPO, Hyoscymus muticus premnaspirodiene oxygenase) is an enzyme with systematic name (-)-vetispiradiene,NADPH:oxygen 2alpha-oxidoreductase.^[1] This enzyme catalyses the following chemical reaction

(-)-vetispiradiene + 2 NADPH + 2 H⁺ + 2 O₂

\rightleftharpoons solavetivone + 2 NADP⁺ + 3 H₂O (overall reaction)

(1a) (-)-vetispiradiene + NADPH + H⁺ + O₂

\rightleftharpoons solavetivol + NADP⁺ + H₂O

(1b) solavetivol + NADPH + H⁺ + O₂

\rightleftharpoons solavetivone + NADP⁺ + 2 H₂O

Premnaspirodiene oxygenase heme-thiolate protein (P-450).

Notes and References

1. Takahashi S, Yeo YS, Zhao Y, O'Maille PE, Greenhagen BT, Noel JP, Coates RM, Chappell J . Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates . The Journal of Biological Chemistry . 282 . 43 . 31744–54 . October 2007 . 17715131 . 2695360 . 10.1074/jbc.M703378200 . free .