Precorrin-8X methylmutase explained

precorrin-8X methylmutase
Ec Number:	5.4.99.61
Go Code:	0016993

In enzymology, a precorrin-8X methylmutase is an enzyme that catalyzes the chemical reaction

precorrin-8X

\rightleftharpoons hydrogenobyrinate

Hence, this enzyme has one substrate, precorrin 8X, and one product, hydrogenobyrinate.

This enzyme belongs to the family of isomerases, specifically those intramolecular transferases transferring other groups. The systematic name of this enzyme class is precorrin-8X 11,12-methylmutase. Other names in common use include precorrin isomerase, hydrogenobyrinic acid-binding protein and CobH. This enzyme is part of the biosynthetic pathway to cobalamin (vitamin B₁₂) in aerobic bacteria.

See also

• Cobalamin biosynthesis

Structural studies

As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes,,,,, and .

References

• Blanche F . 1992 . The final step in the biosynthesis of hydrogenobyrinic acid is catalyzed by the cobH gene product with precorrin-8x as the substrate . J. Bacteriol. . 174 . 1043 - 9 . 1732194 . Couder . M . Famechon . A . Debussche . L . Cameron . B . Crouzet . J . Blanche . F . 3 . 10.1128/JB.174.3.1043-1049.1992 . 206186 .
• Roth JR, Lawrence JG, Rubenfield M, Kieffer-Higgins S, Church GM . 1993 . Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium . J. Bacteriol. . 175 . 3303 - 16 . 8501034 . 11 . 204727 . 10.1128/jb.175.11.3303-3316.1993.
• Stolowich NJ, Iida K, Scott AI . 1992 . Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis. Identification of the 11-methyl and 20-methyl transferases of corrin biosynthesis . FEBS Lett. . 301 . 73 - 8 . 1451790 . 10.1016/0014-5793(92)80213-Z . 1 . 20198692 . free .
• Thibaut D, Debussche L . 1990 . Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid . J. Bacteriol. . 172 . 5980 - 90 . 2211521 . 10 . 10.1128/JB.172.10.5980-5990.1990 . 526920 .