| Poilly-sur-Serein | |
| Commune Status: | Commune |
| Image Coat Of Arms: | Blason de la ville de Poilly-sur-Serein (Yonne).svg |
| Coordinates: | 47.7653°N 3.8933°W |
| Insee: | 89303 |
| Postal Code: | 89310 |
| Arrondissement: | Auxerre |
| Canton: | Chablis |
| Mayor: | Hélène Comoy[1] |
| Term: | 2020 - 2026 |
| Elevation Min M: | 142 |
| Elevation Max M: | 288 |
| Area Km2: | 21.28 |
Poilly-sur-Serein (pronounced as /fr/, literally Poilly on Serein) is a commune in the Yonne department in Bourgogne-Franche-Comté in north-central France.