Plaque hybridization is a technique used in Molecular biology for the identification of recombinant phages.[1] The procedure can also be used for the detection of differentially represented repetitive DNA. The technique (similar to colony hybridization) involves hybridizing isolated phage DNA to a label probe for the gene of study. This is followed by autoradiography to detect the position of the label.[2] The plaque hybridization procedure has some advantages over colony hybridization due to the smaller and well defined area of the filter to which the DNA binds.[3]