PKN2 explained

Serine/threonine-protein kinase N2 is an enzyme that in humans and Strongylocentrotus purpuratus is encoded by the PKN2 gene.^{[1] [2] [3]}

Interactions

PKN2 has been shown to interact with:

• AKT1,^[4]
• NCK1,^{[5] [6]}
• PTPN13,^[7]
• Phosphoinositide-dependent kinase-1,^{[8] [9]} and
• RHOA.^{[5] [10]}

Further reading

• Quilliam LA, Lambert QT, Mickelson-Young LA, Westwick JK, Sparks AB, Kay BK, Jenkins NA, Gilbert DJ, Copeland NG, Der CJ . Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and potential effector of Rho protein signaling . J. Biol. Chem. . 271 . 46 . 28772–28776 . 1997 . 8910519 . 10.1074/jbc.271.46.28772 . free .
• Yu W, Liu J, Morrice NA, Wettenhall RE . Isolation and characterization of a structural homologue of human PRK2 from rat liver. Distinguishing substrate and lipid activator specificities . J. Biol. Chem. . 272 . 15 . 10030–10034 . 1997 . 9092545 . 10.1074/jbc.272.15.10030 . free .
• Vincent S, Settleman J . The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization . Mol. Cell. Biol. . 17 . 4 . 2247–56 . 1997 . 9121475 . 232074 . 10.1128/MCB.17.4.2247.
• Cryns VL, Byun Y, Rana A, Mellor H, Lustig KD, Ghanem L, Parker PJ, Kirschner MW, Yuan J . Specific proteolysis of the kinase protein kinase C-related kinase 2 by caspase-3 during apoptosis. Identification by a novel, small pool expression cloning strategy . J. Biol. Chem. . 272 . 47 . 29449–29453 . 1997 . 9368003 . 10.1074/jbc.272.47.29449 . free .
• Braverman LE, Quilliam LA . Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck . J. Biol. Chem. . 274 . 9 . 5542–5549 . 1999 . 10026169 . 10.1074/jbc.274.9.5542 . free .
• Flynn P, Mellor H, Casamassima A, Parker PJ . Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase . J. Biol. Chem. . 275 . 15 . 11064–11070 . 2000 . 10753910 . 10.1074/jbc.275.15.11064 . free .
• Sun W, Vincent S, Settleman J, Johnson GL . MEK kinase 2 binds and activates protein kinase C-related kinase 2. Bifurcation of kinase regulatory pathways at the level of an MAPK kinase kinase . J. Biol. Chem. . 275 . 32 . 24421–24428 . 2000 . 10818102 . 10.1074/jbc.M003148200 . free .
• Koh H, Lee KH, Kim D, Kim S, Kim JW, Chung J . Inhibition of Akt and its anti-apoptotic activities by tumor necrosis factor-induced protein kinase C-related kinase 2 (PRK2) cleavage . J. Biol. Chem. . 275 . 44 . 34451–34458 . 2000 . 10926925 . 10.1074/jbc.M001753200 . free .
• Gross C, Heumann R, Erdmann KS . The protein kinase C-related kinase PRK2 interacts with the protein tyrosine phosphatase PTP-BL via a novel PDZ domain binding motif . FEBS Lett. . 496 . 2–3 . 101–104 . 2001 . 11356191 . 10.1016/S0014-5793(01)02401-2 . free .
• Hodgkinson CP, Sale GJ . Regulation of both PDK1 and the phosphorylation of PKC-zeta and -delta by a C-terminal PRK2 fragment . Biochemistry . 41 . 2 . 561–569 . 2002 . 11781095 . 10.1021/bi010719z .
• McDonald C, Vacratsis PO, Bliska JB, Dixon JE . The yersinia virulence factor YopM forms a novel protein complex with two cellular kinases . J. Biol. Chem. . 278 . 20 . 18514–18523 . 2003 . 12626518 . 10.1074/jbc.M301226200 . free .
• Anderson NL, Polanski M, Pieper R, Gatlin T, Tirumalai RS, Conrads TP, Veenstra TD, Adkins JN, Pounds JG, Fagan R, Lobley A . The human plasma proteome: a nonredundant list developed by combination of four separate sources . Mol. Cell. Proteomics . 3 . 4 . 311–326 . 2004 . 14718574 . 10.1074/mcp.M300127-MCP200 . free .
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–12135 . 2004 . 15302935 . 514446 . 10.1073/pnas.0404720101 . 2004PNAS..10112130B . free .
• Yarrow JC, Totsukawa G, Charras GT, Mitchison TJ . Screening for cell migration inhibitors via automated microscopy reveals a Rho-kinase inhibitor . Chem. Biol. . 12 . 3 . 385–395 . 2005 . 15797222 . 10.1016/j.chembiol.2005.01.015 . free .
• DeGiorgis JA, Jaffe H, Moreira JE, Carlotti CG, Leite JP, Pant HC, Dosemeci A . Phosphoproteomic analysis of synaptosomes from human cerebral cortex . J. Proteome Res. . 4 . 2 . 306–315 . 2005 . 15822905 . 10.1021/pr0498436 .
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S . Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes . Genome Res. . 16 . 1 . 55–65 . 2006 . 16344560 . 1356129 . 10.1101/gr.4039406 .

Notes and References

1. Palmer RH, Ridden J, Parker PJ . Identification of multiple, novel, protein kinase C-related gene products . FEBS Lett . 356 . 1 . 5–8 . January 1995 . 7988719 . 10.1016/0014-5793(94)01202-4 . free .
2. Palmer RH, Ridden J, Parker PJ . Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family . Eur J Biochem . 227 . 1–2 . 344–351 . March 1995 . 7851406 . 10.1111/j.1432-1033.1995.tb20395.x . free .
3. Web site: Entrez Gene: PKN2 protein kinase N2.
4. Koh H, Lee KH, Kim D, Kim S, Kim JW, Chung J . Inhibition of Akt and its anti-apoptotic activities by tumor necrosis factor-induced protein kinase C-related kinase 2 (PRK2) cleavage . J. Biol. Chem. . 275 . 44 . 34451–8 . November 2000 . 10926925 . 10.1074/jbc.M001753200 . free .
5. Quilliam LA, Lambert QT, Mickelson-Young LA, Westwick JK, Sparks AB, Kay BK, Jenkins NA, Gilbert DJ, Copeland NG, Der CJ . Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and potential effector of Rho protein signaling . J. Biol. Chem. . 271 . 46 . 28772–6 . November 1996 . 8910519 . 10.1074/jbc.271.46.28772. free .
6. Braverman LE, Quilliam LA . Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck . J. Biol. Chem. . 274 . 9 . 5542–9 . February 1999 . 10026169 . 10.1074/jbc.274.9.5542. free .
7. Gross C, Heumann R, Erdmann KS . The protein kinase C-related kinase PRK2 interacts with the protein tyrosine phosphatase PTP-BL via a novel PDZ domain binding motif . FEBS Lett. . 496 . 2–3 . 101–4 . May 2001 . 11356191 . 10.1016/s0014-5793(01)02401-2. free .
8. Hodgkinson CP, Sale GJ . Regulation of both PDK1 and the phosphorylation of PKC-zeta and -delta by a C-terminal PRK2 fragment . Biochemistry . 41 . 2 . 561–9 . January 2002 . 11781095 . 10.1021/bi010719z.
9. Balendran A, Biondi RM, Cheung PC, Casamayor A, Deak M, Alessi DR . A 3-phosphoinositide-dependent protein kinase-1 (PDK1) docking site is required for the phosphorylation of protein kinase Czeta (PKCzeta) and PKC-related kinase 2 by PDK1 . J. Biol. Chem. . 275 . 27 . 20806–13 . July 2000 . 10764742 . 10.1074/jbc.M000421200 . free .
10. Flynn P, Mellor H, Palmer R, Panayotou G, Parker PJ . Multiple interactions of PRK1 with RhoA. Functional assignment of the Hr1 repeat motif . J. Biol. Chem. . 273 . 5 . 2698–705 . January 1998 . 9446575 . 10.1074/jbc.273.5.2698. free .