| Pixley National Wildlife Refuge | |
| Iucn Category: | IV |
| Map: | USA |
| Relief: | 1 |
| Map Width: | 300 |
| Location: | Tulare County, California, United States |
| Nearest City: | Pixley, California |
| Coordinates: | 35.9172°N -119.3623°W |
| Area: | 6939acres |
| Established: | 1959 |
| Governing Body: | U.S. Fish and Wildlife Service |
| Website: | Pixley National Wildlife Refuge |
Pixley National Wildlife Refuge is located south of Tulare, California and north of Bakersfield in the San Joaquin Valley. The 6939acres nature refuge represents one of the few remaining examples of the grasslands, vernal pools, and playas that once bordered historic Tulare Lake, the largest lake west of the Great Lakes until the late 19th century.
Over 100 bird and 6 reptile species use the wildlife refuge. Approximately of managed wetlands provide habitat for migratory waterfowl and shorebirds. Threatened and endangered species include the San Joaquin kit fox, blunt-nosed leopard lizard, Tipton kangaroo rat, and the vernal pool fairy shrimp.
Refuge visitation is by special arrangement only.