Pimelyl-(acyl-carrier protein) methyl ester esterase explained

Pimelyl-[acyl-carrier protein] methyl ester esterase
Ec Number:	3.1.1.85

The enzyme Pimelyl-[acyl-carrier protein] methyl ester esterase (EC 3.1.1.85, BioH; systematic name pimelyl-[acyl-carrier protein] methyl ester hydrolase^{[1] [2] [3] [4]} catalyses the reaction

pimelyl-[acyl-carrier protein] methyl ester + H₂O

\rightleftharpoons pimelyl-[acyl-carrier protein] + methanol

This enzyme takes part in biotin biosynthesis in Gram-negative bacteria.

Notes and References

1. Sanishvili R, Yakunin AF, Laskowski RA, Skarina T, Evdokimova E, Doherty-Kirby A, Lajoie GA, Thornton JM, Arrowsmith CH, Savchenko A, Joachimiak A, Edwards AM . Integrating structure, bioinformatics, and enzymology to discover function: BioH, a new carboxylesterase from Escherichia coli . The Journal of Biological Chemistry . 278 . 28 . 26039–45 . July 2003 . 12732651 . 2792009 . 10.1074/jbc.M303867200 . free .
2. Lemoine Y, Wach A, Jeltsch JM . To be free or not: the fate of pimelate in Bacillus sphaericus and in Escherichia coli . Molecular Microbiology . 19 . 3 . 645–7 . February 1996 . 8830257 . 10.1046/j.1365-2958.1996.t01-4-442924.x .
3. Tomczyk NH, Nettleship JE, Baxter RL, Crichton HJ, Webster SP, Campopiano DJ . Purification and characterisation of the BIOH protein from the biotin biosynthetic pathway . FEBS Letters . 513 . 2–3 . 299–304 . February 2002 . 11904168 . 10.1016/s0014-5793(02)02342-6 . free .
4. Lin S, Hanson RE, Cronan JE . Biotin synthesis begins by hijacking the fatty acid synthetic pathway . Nature Chemical Biology . 6 . 9 . 682–8 . September 2010 . 20693992 . 2925990 . 10.1038/nchembio.420 .