Phikmvvirus Explained

Phikmvvirus is a genus of viruses that infect bacteria. There are currently 16 species in this genus including the type species Pseudomonas virus phiKMV.[1] [2] Bacteriophage phiKMV[3] and its relatives are known to be highly virulent phages, producing large (3mm15mm diameter) clear plaques on a susceptible host.[4] [5] The only reported exception is phage LKA1, which yields small plaques (1mm) surrounded by a halo. While all other P. aeruginosa-specific phikmvviruses use the Type IV pili as primary receptor, LKA1 particles attach to the bacterial lipopolysaccharide layer.

Taxonomy

The following species are recognized:

Virology

Electron microscopic imaging of purified phage particles revealed these phages as typical members of the Podoviridae, with a head diameter of approximately 60nm and a stubby tail with a length of 8–10 nm. Although phiKMV phage resembles the well-studied podovirus T7 in overall genome architecture, it was the first known T7-like phage which encoded a single-subunit RNA polymerase gene downstream its DNA metabolism genes instead of in the early genomic region. Based on these properties, the genus Phikmvvirus is classified within the Autographiviridae.[6]

Genus Structure Symmetry !Capsid Genomic arrangement Genomic segmentation
PhikmvvirusHead-TailT=7Non-envelopedLinearMonopartite

Life cycle

Viral replication is cytoplasmic. Entry into the host cell is achieved by absorption into the host cell. DNA-templated transcription is the method of transcription. The virus exits the host cell by lysis, and holin/endolysin/spanin proteins. Bacteria serve as the natural host. Transmission routes are passive diffusion.

External links

Notes and References

  1. Web site: Viral Zone. ExPASy. 15 June 2015.
  2. Web site: Virus Taxonomy: 2019 Release. talk.ictvonline.org. International Committee on Taxonomy of Viruses. 4 May 2020.
  3. Lavigne . Rob . Burkal'tseva . Maria V. . Robben . Johan . Sykilinda . Nina N. . Kurochkina . Lidia P. . Grymonprez . Barbara . Jonckx . Bart . Krylov . Victor N. . Mesyanzhinov . Vadim V. . Volckaert . Guido . The genome of bacteriophage φKMV, a T7-like virus infecting Pseudomonas aeruginosa . Virology . 312 . 1 . 49–59 . 2003 . 12890620 . 10.1016/S0042-6822(03)00123-5. 8 . free .
  4. Ceyssens . P.-J. . Lavigne . R. . Mattheus . W. . Chibeu . A. . Hertveldt . K. . Mast . J. . Robben . J. . Volckaert . G. . Genomic Analysis of Pseudomonas aeruginosa Phages LKD16 and LKA1: Establishment of the KMV Subgroup within the T7 Supergroup . Journal of Bacteriology . 188 . 19 . 6924–31 . 2006 . 16980495 . 1595506 . 10.1128/JB.00831-06.
  5. Lammens . E. . Ceyssens . P.-J. . Voet . M. . Hertveldt . K. . Lavigne . R. . Volckaert . G. . Representational Difference Analysis (RDA) of bacteriophage genomes . Journal of Microbiological Methods . 77 . 2 . 207–13 . 2009. 19232531 . 10.1016/j.mimet.2009.02.006.
  6. Lavigne . Rob . Seto . Donald . Mahadevan . Padmanabhan . Ackermann . Hans-W. . Kropinski . Andrew M. . Unifying classical and molecular taxonomic classification: analysis of the Podoviridae using BLASTP-based tools . Research in Microbiology . 159 . 5 . 406–14 . 2008 . 18555669 . 10.1016/j.resmic.2008.03.005. free .

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Phikmvvirus".

Except where otherwise indicated, Everything.Explained.Today is © Copyright 2009-2024, A B Cryer, All Rights Reserved. Cookie policy.