Peroxyureidoacrylate/ureidoacrylate amidohydrolase explained

Peroxyureidoacrylate/ureidoacrylate amidohydrolase
Ec Number:	3.5.1.110

Peroxyureidoacrylate/ureidoacrylate amidohydrolase (RutB) is an enzyme with systematic name (Z)-3-ureidoacrylate peracid amidohydrolase.^[1] This enzyme catalyses the following chemical reaction sequences:

• (Z)-3-ureidoacrylate peracid + H₂O ⇌ (Z)-3-peroxyaminoacrylate + CO₂ + NH₃ (overall reaction)
  1. (Z)-3-ureidoacrylate peracid + H₂O ⇌ (Z)-3-peroxyaminoacrylate + carbamate
  2. carbamate ⇌ CO₂ + NH₃ (spontaneous)
• (Z)-2-methylureidoacrylate peracid + H₂O ⇌ (Z)-2-methylperoxyaminoacrylate + CO₂ + NH₃ (overall reaction)
  1. (Z)-2-methylureidoacrylate peracid + H₂O ⇌ (Z)-2-methylperoxyaminoacrylate + carbamate
  2. carbamate ⇌ CO₂ + NH₃ (spontaneous)

The enzyme also shows activity towards ureidoacrylate.

Notes and References

1. Kim KS, Pelton JG, Inwood WB, Andersen U, Kustu S, Wemmer DE . The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems . Journal of Bacteriology . 192 . 16 . 4089–102 . August 2010 . 20400551 . 2916427 . 10.1128/JB.00201-10 .