| Pernille Holst Holmsgaard | |
| Nationality: | Danish |
| Birth Date: | 6 September 1984 |
| Birth Place: | Pandrup, Denmark |
| Position: | Left back |
| Currentclub: | retired |
| Clubs1: | Jetsmark HK |
| Years2: | –2007 |
| Clubs2: | Horsens HK |
| Years3: | 2007–2009 |
| Clubs3: | Aalborg DH |
| Years4: | 2009–2011 |
| Clubs4: | HC Odense |
| Years5: | 2011–2013 |
| Clubs5: | Viborg HK |
| Years6: | 2013–2016 |
| Clubs6: | København Håndbold |
| Years7: | 2016-2017 |
| Clubs7: | Nykøbing F. Håndboldklub |
| Nationalyears1: | 2006–2017 |
| Nationalteam1: | Denmark |
| Nationalcaps1: | 136 |
| Nationalgoals1: | 124 |
Pernille Holst Holmsgaard (born 6 September 1984) is a former Danish handball player, who last played for Nykøbing Falster Håndboldklub and for the Danish women's national handball team.
At the 2010 European Women's Handball Championship she reached the bronze final and placed fourth with the Danish team.[1]