Pentalenene oxygenase explained

Pentalenene oxygenase
Ec Number:	1.14.15.32

Pentalenene oxygenase (Formerly, PtlI) is an enzyme with systematic name pentalenene,NADPH:oxygen 13-oxidoreductase.^[1] This enzyme catalyses the following chemical reaction

pentalenene + 2 NADPH + 2 H⁺ + 2 O₂

\rightleftharpoons pentalen-13-al + 2 NADP⁺ + 3 H₂O (overall reaction)

(1a) pentalenene + NADPH + H⁺ + O₂

\rightleftharpoons pentalen-13-ol + NADP⁺ + H₂O

(1b) pentalen-13-ol + NADPH + H⁺ + O₂

\rightleftharpoons pentalen-13-al + NADP⁺ + 2 H₂O

Pentalenene oxygenase is a heme-thiolate protein (P-450).

Notes and References

1. Quaderer R, Omura S, Ikeda H, Cane DE . Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlI, a cytochrome P450 of Streptomyces avermitilis . Journal of the American Chemical Society . 128 . 40 . 13036–7 . October 2006 . 17017767 . 2533730 . 10.1021/ja0639214 .