Pectate trisaccharide-lyase explained

Pectate trisaccharide-lyase
Ec Number:	4.2.2.22

Pectate trisaccharide-lyase (exopectate-lyase, pectate lyase A, PelA) is an enzyme with systematic name (1→4)-α-D-galacturonan reducing-end-trisaccharide-lyase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction:

eliminative cleavage of unsaturated trigalacturonate as the major product from the reducing end of polygalacturonic acid/pectate

The predominant action of this enzyme is removal of a trisaccharide.

Notes and References

1. Kluskens LD, van Alebeek GJ, Voragen AG, de Vos WM, van der Oost J . Molecular and biochemical characterization of the thermoactive family 1 pectate lyase from the hyperthermophilic bacterium Thermotoga maritima . The Biochemical Journal . 370 . Pt 2 . 651–9 . March 2003 . 12443532 . 1223193 . 10.1042/bj20021595 .
2. Tamaru Y, Doi RH . Pectate lyase A, an enzymatic subunit of the Clostridium cellulovorans cellulosome . Proceedings of the National Academy of Sciences of the United States of America . 98 . 7 . 4125–9 . March 2001 . 11259664 . 31190 . 10.1073/pnas.071045598 . free .
3. Berensmeier S, Singh SA, Meens J, Buchholz K . Cloning of the pelA gene from Bacillus licheniformis 14A and biochemical characterization of recombinant, thermostable, high-alkaline pectate lyase . Applied Microbiology and Biotechnology . 64 . 4 . 560–7 . May 2004 . 14673544 . 10.1007/s00253-003-1446-9 . 23236787 .