| Pandy's test | |
| Purpose: | detect the elevated levels of proteins |
Pandy's test (or Pandy's reaction) is done on the CSF (cerebrospinal fluid) to detect the elevated levels of proteins (mainly globulins). This test is named after the Hungarian neurologist, Pándy Kálmán (1868–1945) who developed this test in the year 1910.
Proteins (globulin and albumin) are precipitated by a saturated solution of phenol in water.
The reagent used is phenol (carbolic acid crystals dissolved in water) or, pyrogallic acid or, cresol, usually termed as Pandy's reagent or Pandy's solution.
One drop of CSF sample (collected from the patient by lumbar puncture technique), is added to about 1ml of Pandy's solution. The turbid appearance signifies the presence of elevated levels of globulin protein in the CSF and is regarded as positive Pandy's reaction. The CSF from a normal adult shows no turbidity or precipitates and this is a negative Pandy's reaction.
Proteins in the cerebrospinal fluid, normally albumin and globulin are present in the ratio of 8 to 1. Increases in protein levels are of diagnostic value in neurological diseases.
The normal CSF is clear and transparent fluid. The Pandy's reaction makes it translucent or opaque.
A positive test shows a bluish-white streak of precipitated proteins. The degree of turbidity depends on the amount of protein in the CSF. It can vary from faint turbidity (mild to moderate elevation in CSF proteins) to dense milky precipitate (high protein content in CSF).
The positive Pandy's reaction may indicate one or more of the following pathological conditions:
No cloudy turbidity observed. The CSF sample is normal i.e. with normal protein contents.
Please note that the normal CSF protein is also obtained in several pathological conditions like viral CNS infections, brainstem glioma, ischemic cerebrovascular accident.
Diagnostic test